期刊
INDUSTRIAL CROPS AND PRODUCTS
卷 186, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.indcrop.2022.115186
关键词
Waste paper; Cellulose; Cellulase; Cellobiose; Cellobionic acid; Pseudomonas taetrolens
资金
- R & D program of MOTIE/KEIT [10 077 291]
- R & D program of KRICT [S2242-10, BSF21-505]
- R & D program of Ulsan-KRICT [US21-12, US21-12-01]
In this study, waste office paper was valorized into cellobionic acid using a two-step biocatalytic method. The researchers successfully optimized the reaction conditions and achieved a high yield of cellobiose and CBA. This study lays the groundwork for low-cost production of CBA and has the potential to expand its application in industries.
To expand the applications of waste paper (WP), we attempted to valorize waste office paper (WOP) into cellobionic acid (CBA) using a two-step biocatalytic method. First, we prepared cellobiose from WOP using commercial cellulases. To increase cellobiose production, we optimized various reaction conditions; the type of cellulases, reaction temperature, the amount of WOP, and the amount of cellulases. Under the optimized reaction conditions, 23 g/L cellobiose was generated from 80 g/L of WOP. CBA was then produced from the prepared cellobiose using recombinant Pseudomonas taetrolens homologously expressing quinoprotein glucose dehydrogenase. From 23 g/L cellobiose derived from WOP, this strain produced 24.1 g/L CBA. We demonstrated for the first time that WOP could be successfully valorized into CBA using cellobiogenic cellulases and a CBA-producing strain. This study sets the groundwork to produce CBA at a low price and is expected to expand the application of CBA in industries.
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