期刊
CARBOHYDRATE POLYMERS
卷 117, 期 -, 页码 807-812出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2014.10.044
关键词
Duckweed; Galactoarabinan; alpha-1,5-Arabinan backbone; beta-1,3-Galactan side chain; Cell wall antibody; Yariv assay
资金
- Director Innovation Foundation of Qingdao Institute of Bioenergy and Bioprocess Technology
- Shandong Agricultural Significant Application of Technology Innovation Program
- China Risun Coal Chemicals Group Limited
A highly branched galactoarabinan named DAG1 (Mw similar to 4.0 x 10(4) Da) was purified from Lemna aequinoctialis 6000 via 70% (v/v) ethanol extraction, followed by size-exclusion chromatography on Bio-Gel P2 and Superdex 75. Methylation analysis showed that DAG1 consisted of t-Araf, (1 -> 5)-Araf, (1 -> 2,5)-Araf, (1 -> 3)-Galp, and (1 -> 3,6)-Galp in a relative proportion of approximately 6:4:3:3:3, suggesting an arabinogalactan/galactoarabinan polysacchairde. With the aid of arabinan degrading enzymes, the structure of DAG1 repeating unit was further characterized by ELISA with specific monoclonal antibodies and Yariv reagent assay. Analyses indicated that the proposed repeating unit of DAG1 had a backbone composed of seven alpha-(1 -> 5)-L-arabinofuranose residues where branching occurred at O-2 with either terminal arabinoses or arabinogalactan side chain. The arabinogalactan side chain was composed of six beta-(1 -> 3)-D-galactopyranose residues, half of which were ramified at O-6 with terminal arabinoses and the last galactose was terminated with arabinose. (C) 2014 Elsevier Ltd. All rights reserved.
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