期刊
HUMAN MUTATION
卷 43, 期 12, 页码 2170-2186出版社
WILEY-HINDAWI
DOI: 10.1002/humu.24482
关键词
genetic testing; juvenile open-angle glaucoma; MYOC; primary open-angle glaucoma; variant classification; variant curation expert panel; variant interpretation
资金
- Hospital Research Foundation
- Research to Prevent Blindness
- National Institutes of Health
- National Health and Medical Research Council
Standardization of variant curation criteria is crucial for accurate interpretation of genetic results and clinical care. This study developed gene-specific variant curation guidelines for myocilin (MYOC) and piloted them on 81 variants, resulting in a change in classification for 40% of the variants in ClinVar. Functional evidence played a role in the reclassification of 18 variants. These guidelines provide a framework for consistent application of rules in laboratories to improve MYOC genetic testing in the management of glaucoma.
The standardization of variant curation criteria is essential for accurate interpretation of genetic results and clinical care of patients. The variant curation guidelines developed by the American College of Medical Genetics and Genomics (ACMG) and the Association for Molecular Pathology (AMP) in 2015 are widely used but are not gene specific. To address this issue, the Clinical Genome Resource (ClinGen) Variant Curation Expert Panels (VCEP) have been tasked with developing gene-specific variant curation guidelines. The Glaucoma VCEP was created to develop rule specifications for genes associated with primary glaucoma, including myocilin (MYOC), the most common cause of Mendelian glaucoma. Of the 28 ACMG/AMP criteria, the Glaucoma VCEP adapted 15 rules to MYOC and determined 13 rules not applicable. Key specifications included determining minor allele frequency thresholds, developing an approach to counting probands and segregations, and reviewing functional assays. The rules were piloted on 81 variants and led to a change in classification in 40% of those that were classified in ClinVar, with functional evidence influencing the classification of 18 variants. The standardized variant curation guidelines for MYOC provide a framework for the consistent application of the rules between laboratories, to improve MYOC genetic testing in the management of glaucoma.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据