期刊
GENOMICS
卷 114, 期 5, 页码 -出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygeno.2022.110442
关键词
miRNA profiles; Mammary gland; Regenerative involution; Dry period
资金
- National Natural Science Foundation of China [31702090]
- Key R&D program of Zhejiang Province [2022C04017]
- Zhejiang Provincial Major Science and Technology Projects on Agricultural New Varieties Selection and Breeding [2021C02068-6]
In this study, two distinct miRNA expression patterns were found to be associated with mammary regenerative involution. Differentially expressed miRNAs were primarily involved in tissue self-renewal and enriched in pathways related to vesicle-mediated transport, tissue development, tube development, vasculature development, and epithelial development.
Regenerative involution is important for the subsequent lactation, but molecular mechanism has not been revealed. The crucial miRNA in tissue development indicates that miRNAs might participate in regenerative involution. In the present study, the mammary tissues of the dairy goats (n = 3) were collected via biopsy at wk-8 (time to dry off), -6, -4, -1, and + 1 relative to lambing for the Hematoxylin and Eosin staining and miRNA sequencing. Alveolar structures collapsed during regenerative involution, but the structures remained intact and distended. Among the 50 miRNA expression trajectories categorized by short time-series expression miner, two significant patterns were clustered. The differentially expressed miRNAs in the two patterns were mainly related to the self-renewal of tissue and enriched in pathways containing vesical-mediated transport, tissue development, tube development, vasculature development and epithelial development. The identification of the miRNAs will help in elucidating their regulatory roles in mammary gland development.
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