期刊
FUEL
卷 325, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.fuel.2022.124844
关键词
3-Oxoacyl acyl carrier protein reductase; Fatty acid; Biofuel; Stress tolerance; Diatom
资金
- Southern Marine Science and Engi-neering Guangdong Laboratory (Guangzhou) [SMSEGL20SC02]
- Natural Science Foundation of China [51908244]
- Characteristic Innovation Projects of Ordinary Colleges and Universities in Guangdong Province [2021KTSCX009]
This study successfully improved lipid and fatty acid biosynthesis in Phaeodactylum tricornutum by introducing the FabG gene, and enhanced the tolerance of transgenic algae to high temperature stress. This method shows great potential for high-quality biodiesel production.
Due to its high lipid accumulation capability, Phaeodactylum tricornutum has attracted considerable attention as a promising candidate for biofuel production. However, the derived lipid cannot satisfy the industrial biofuel manufacturing requirements given its poor fatty acid characteristics. Genetic engineering, as a prospective approach, has been employed to overcome this bottleneck. Relevant research indicates that the fabG gene plays a crucial role in fatty acid biosynthesis in bacteria, but its significance in microalgae remains unclear. In this study, FabG was successfully introduced, transcribed, and overexpressed in P. tricornutum. FabG overexpression significantly enhanced lipid (1.49-fold) and fatty acid (1.31-fold) biosynthesis without altering growth and photosynthesis, and this method thus exhibits great potential in high-quality biodiesel production. Moreover, increased saturation of fatty acids (e.g., C16:0) and improved antioxidative activities enhanced the tolerance of transgenic P. tricornutum to high temperature stress. To conclude, this study reports a feasible approach for high quality algae-based biofuel production via the unprecedented roles of FabG retrieved from diatom.
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