期刊
FOOD CHEMISTRY
卷 390, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2022.133188
关键词
Self-aggregated gold nanoparticles; Opium poppy; Morphine; Codeine; Thebaine; Herbal teas
资金
- National Scientific Foundation of China [31871883, 21874048]
- HeYuan Planned Program in Science and Technology [2019041]
- Generic Technique Innovation Team Construction of Modern Agriculture of Guangdong Province [2021KJ130]
- National Key Research and Development Program of Thirteenth Five-Year Plan [2017YFC1601700]
In this study, an easily prepared highly aggregated gold nanoparticles (AGNPs) and its application in lateral flow immunoassay (LFIA) for detecting opium poppy abuse in herbal teas were developed. The method showed rapid, sensitive, and accurate detection.
Opium poppy abused in food has aroused public concerns due to its serious side effects. Effective monitoring is essential to fight the abuse crisis. Herein, we synthesized an easily prepared, affordable, accessible highly aggregated gold nanoparticles (AGNPs) performing in lateral flow immunoassay (LFIA) for detection opium poppy in herbal teas. Simultaneously, a LFIA based on time-resolved fluorescent microspheres (TRFMs) was developed as contrastive method. In this study, morphine (MOR), codeine (COD) and thebaine (THE) were as the specific recognition markers of opium poppy. Results demonstrated the quantitative limits of detection were 0.0049/0.0053/0.084, 0.034/0.037/0.37 ng mL-1 for AGNPs/TRFMs-LFIA, respectively. The recoveries were 95%-107.5%/91%-106.7% with coefficient of variation was 1.6%-6.6%/1.8%-7.2%, indicating excellent accuracy and precision. Parallel experiments among AGNPs/TRFMs-LFIA and LC-MS/MS analysis showed good correlation. Overall, AGNPs-LFIA executed quantitative analysis within 15 min on the basis of simple treatment while providing a rapid and sensitive analysis strategy for illegal drugs abused.
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