Specifically triggered dissociation based ratiometric electrochemical sensor for H2O2 measurement in food samples

A novel ratiometric strategy based electrochemical sensor was developed to quantitative assay of H2O2 in different food samples. 4-aminophenylboronic acid pinacol ester (ABAPE) dissociation was specifically triggered by H2O2 to generate electro-active 4-aminophenol (4-AP), which not only can be oxidized to indirectly indicate the concentration of H2O2, but also endowed the sensor with high selectivity. Meanwhile, a reference probe of poly(thionine) (TH) was modified with ketjen black (KB) and gold nanoparticles (AuNPs) on electrode surface. KB and AuNPs displayed high electrocatalytic activity to 4-AP. A current ratio between 4-AP and TH (i/i(TH)) showed a good linear relationship with the concentration of H2O2 in a range of 3.0 x 10(-7) - 1.0 x 10(-4) mol/L (0.010 ppm - 3.40 ppm) with a limit of detection of 2.6 x 10(-7) mol/L (0.009 ppm) (S/N = 3). Moreover, the ratiometric strategy based sensor possessed good accuracy, reliability, and stability, and successfully determined H2O2 in food samples with satisfactory results.

Automated summary

A novel ratiometric strategy-based electrochemical sensor has been developed for quantitative determination of H2O2 in different food samples. The sensor exhibits high selectivity and accuracy, and has been successfully applied for H2O2 determination in food samples.