期刊
FISH & SHELLFISH IMMUNOLOGY
卷 129, 期 -, 页码 114-126出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2022.08.049
关键词
Oreochromis niloticus; Complement factor H; Bacterial infection; Phagocytosis; Inflammatory response
资金
- National Natural Science Foundation of China
- Guangzhou Basic and Applied Basic Research Project
- Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams
- [31972818]
- [202102020905]
- [2019KJ141]
The study demonstrates that CFH in Nile tilapia can bind to pathogens and play a role in immune response against bacterial infection.
Complement factor H (CFH), a multifunctional soluble complement regulatory protein, can bind to a variety of pathogens and play a crucial role in host innate immune defense. To explore the functional characteristics of CFH (OnCFH) in Nile tilapia (Oreochromis niloticus), we cloned and characterized the open reading frame (ORF) of OnCFH in this study. The full-length of OnCFH ORF is 1359 bp, encoding 452 aa for a 48.85 kDa peptide, and its predicted structure containing six short complement-like repeats (SCRs). The analysis of tissue distribution showed that OnCFH was constitutively expressed in all tested tissues, with the highest in the liver. Upon Streptococcus agalactiae and Aeromonas hydrophila stimuli in vivo and in vitro, OnCFH mRNA transcript was significantly upregulated in head kidney tissue as well as head kidney monocytes/macrophages. Further, the recombinant OnCFH protein ((r)OnCFH) could bind to pathogenic bacteria in a dose-dependent. Moreover, it got involved in the regulation of inflammation as well as phagocytosis of monocytes/macrophages. The knockdown of OnCFH remarkably decreased the amount of bacteria in the head kidney. In summary, our data demonstrated that OnCFH could participate in the immune response of Nile tilapia against bacterial infection.
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