期刊
EXPERIMENTAL CELL RESEARCH
卷 420, 期 1, 页码 -出版社
ELSEVIER INC
DOI: 10.1016/j.yexcr.2022.113337
关键词
CTP synthase; Cytoophidium; Ubiquitination; Filament; Schizosaccharomyces pombe
资金
- Ministry of Science and Technology of China [2021YFA0804700]
- National Natural Science Foundation of China [31771490]
- Shanghai Science and Technology Commission [20JC1410500]
- ShanghaiTech University
- UK Medical Research Council [MC_UU_12,021/3, MC_U137788471]
In this study, it was found that ubiquitination plays an important role in regulating the formation of cytoophidia. The proteins associated with CTPS and the ubiquitination regulators that significantly affect CTPS filamentation were identified. Additionally, a group of deubiquitinating enzymes were found to be important for regulating the filamentous morphology of cytoophidia.
CTP synthase (CTPS), a metabolic enzyme responsible for the de novo synthesis of CTP, can form filamentous structures termed cytoophidia, which are evolutionarily conserved from bacteria to humans. Here we used Schizosaccharomyces pombe to study the cytoophidium assembly regulation by ubiquitination. We tested the CTP synthase's capacity to be post-translationally modified by ubiquitin or be affected by the ubiquitination state of the cell and showed that ubiquitination is important for the maintenance of the CTPS filamentous structure in fission yeast. We have identified proteins which are in complex with CTPS, including specific ubiquitination regulators which significantly affect CTPS filamentation, and mapped probable ubiquitination targets on CTPS. Furthermore, we discovered that a cohort of deubiquitinating enzymes is important for the regulation of cytoophidium's filamentous morphology. Our study provides a framework for the analysis of the effects that ubiquitination and deubiquitination have on the formation of cytoophidia.
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