期刊
EXPERIMENTAL AND APPLIED ACAROLOGY
卷 88, 期 1, 页码 41-55出版社
SPRINGER
DOI: 10.1007/s10493-022-00744-5
关键词
Atopic dermatitis; Canine IgE; Molecular diagnosis; House dust mite; Storage mite; Recombinant proteins
类别
资金
- Medical Device Technology Development Program [200006057]
- Ministry of Trade, Industry and Energy (MOTIE, Korea)
House dust mite is a common cause of atopic dermatitis in humans and dogs. Detection of serum IgE to allergens is commonly used for diagnosis, but cross-reactivity and non-specific reactivity can lead to misdiagnosis. By comparing human and canine IgE reactivities to mite component allergens, different sensitization patterns were found between allergic humans and dogs.
House dust mite is a common cause of atopic dermatitis (AD) both in humans and dogs. Detection of serum IgE to allergens is commonly used to diagnose allergic diseases. However, false-positive reactions due to cross-reactivity and non-specific reactivity may lead to misdiagnosis. We compared human and canine IgE reactivities to mite component allergens. Canine IgE-reactive components of Dermatophagoides farinae and Tyrophagus putrescentiae were identified by tandem mass spectrometry. Recombinant proteins were produced and IgE reactivities to component allergens were assessed by ELISA and inhibition assays using sera from AD patients and dogs. Canine IgE-reactive proteins (Der f 1, Der f 11, Tyr p 4, Tyr p 8, Tyr p 11, Tyr p 28) were identified by proteome analysis. Most patients were sensitized to Der f 1 (93.3%) and Der f 2 (86.7%). Dogs showed high sensitization to Der f 2 (94.1%) and Der f 18 (84.6%). Both patients and dogs showed low IgE binding frequency to Tyr p 8, 43.3% and 4%, respectively. The ELISA inhibition study indicated that canine IgE reactivity to T. putrescentiae is mostly due to non-specific reaction and cross-reaction with D. farinae. Different IgE sensitization patterns were shown between allergic humans and dogs with AD, especially to Der f 18, for the first time in Korea. Furthermore, non-specific canine IgE reactivity to storage mite indicates the possibility of misdiagnoses. Standardizations focused on the major canine allergen content of extracts should be developed. This will allow precision diagnosis and individuated treatments for each patient and atopic dog.
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