期刊
EUROPEAN UROLOGY
卷 82, 期 6, 页码 602-610出版社
ELSEVIER
DOI: 10.1016/j.eururo.2022.08.004
关键词
Clinical trial; Bacillus Calmette-Guerin; Digital spatial profiling; Bladder cancer; Intravesical pembrolizumab
资金
- MISP
- Robert H. Lurie Cancer Center
- Northwestern University RHLCCC Flow Cytometry Facility
- VHA [BX005599, BX003692]
- Cancer Center Support grant [NCI CA060553]
Intravesical pembrolizumab combined with BCG is a safe and feasible treatment option for bacillus Calmette-Guerin (BCG)-unresponsive non-muscle-invasive bladder cancer, capable of eliciting strong immune responses.
Background: Intravenous immune checkpoint inhibition is an effective anticancer strategy for bacillus Calmette-Guerin (BCG)-unresponsive non-muscle-invasive bladder cancer (NMIBC) but may be associated with greater systemic toxicity compared with localized therapies. Objective: We assessed the safety and antitumor activity of intravesical pembrolizumab combined with BCG. Design, setting, and participants: A 3 + 3 phase 1 trial of pembrolizumab + BCG was conducted in patients with BCG-unresponsive NMIBC (NCT02808143). Intervention: Pembrolizumab was given intravesically (1-5 mg/kg for 2 h) beginning 2 weeks prior to BCG induction until recurrence. Urine profiling during treatment and spatial transcriptomic profiling of pre- and post-treatment tumors were conducted to identify biomarkers that correlated with response. Outcome measurements and statistical analysis: Safety and tolerability of immune checkpoint inhibition were assessed, and Kaplan-Meier survival analysis was performed. Results and limitations: Nine patients completed therapy. Median follow-up was 35 months for five patients still alive at the end of the trial. The trial was closed due to the COVID-19 pandemic. Grade 1-2 urinary symptoms were common. The maximum tolerated dose was not reached; however, one dose-limiting toxicity was reported (grade 2 diarrhea) in the only patient who reached 52 weeks without recurrence. One death occurred from myasthenia gravis that was deemed potentially related to treatment. The 6-mo and 1-yr recurrence-free rates were 67% (95% confidence interval [CI]: 42-100%) and 22% (95% CI: 6.5-75%), respectively. Pembrolizumab was detected in the urine and not in blood. CD4(+) T cells were significantly increased in the urine after treatment, and a transcriptomic analysis identified decreased expression of T-cell exhaustion markers in late recurrences. Conclusions: We demonstrate that intravesical pembrolizumab is safe, feasible, and capable of eliciting strong immune responses in a clinical setting and should be investigated further. Patient summary: Direct application of pembrolizumab to the bladder is a promising alternative for non-muscle-invasive bladder cancer unresponsive to Bacillus Calmette-Guerin and should be investigated further. (c) 2022 The Author(s). Published by Elsevier B.V. on behalf of European Association of Urology.
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