4.6 Review

A precise review on NAATs-based diagnostic assays for COVID-19: A motion in fast POC molecular tests

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Medicine, General & Internal

Evaluation of real-time NASBA assay for the detection of SARS-CoV-2 compared with real-time PCR

Vahid Kia et al.

Summary: The developed real-time NASBA assay in this study is a sensitive and specific method for the detection of SARS-CoV-2, comparable to real-time PCR. The limit of detection of the assay is 200 copies/mL, and the clinical sensitivity is 97.64%. Compared to other isothermal methods, real-time NASBA has a straightforward primer design and can be used for inexpensive detection.

IRISH JOURNAL OF MEDICAL SCIENCE (2023)

Review Medical Laboratory Technology

CRISPR-based tools: Alternative methods for the diagnosis of COVID-19

Fahreddin Palaz et al.

Summary: Detection of the SARS-CoV-2 virus is crucial in combating the global pandemic, with RT-qPCR being the gold standard method but having limitations in practical implementation. Recently developed CRISPR-based detection methods offer rapid and cost-effective platforms with high sensitivity and specificity.

CLINICAL BIOCHEMISTRY (2021)

Article Infectious Diseases

Comparison of diagnostic performance of five molecular assays for detection of SARS-CoV-2

Neena Kanwar et al.

Summary: The study compared the performance of five different SARS-CoV-2 detection assays and found that Aptima and Abbott assays had the highest positive percent agreement, while the BGI assay had the lowest agreement with 10 missed detections. Negative percent agreement for all 5 platforms was comparable, ranging from 96.1% to 100%. The performance of all five assays was comparable.

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE (2021)

Article Infectious Diseases

SARS-CoV-2 N gene mutations impact detection by clinical molecular diagnostics: reports in two cities in the United States

Mindy Leelawong et al.

Summary: Nasal and nasopharyngeal swab specimens tested by Cepheid Xpert Xpress SARS-CoV-2 were analyzed by whole-genome sequencing based on impaired detection of the N2 target. Each viral genome had at least one mutation in the N gene, which likely arose independently in the New York City and Pittsburgh study sites.

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE (2021)

Article Chemistry, Analytical

Rapid One-Pot Detection of SARS-CoV-2 Based on a Lateral Flow Assay in Clinical Samples

Chao Zhang et al.

Summary: This study developed a rapid detection method for SARS-CoV-2, with the entire sample-to-result workflow taking less than 40 minutes. The method achieved 100% accuracy compared to PCR-based assays, offering a potential universal platform for rapid and point-of-care detection of emerging infectious diseases.

ANALYTICAL CHEMISTRY (2021)

Article Chemistry, Analytical

Reverse Transcription Recombinase Polymerase Amplification Coupled with CRISPR-Cas12a for Facile and Highly Sensitive Colorimetric SARS-CoV-2 Detection

Wei S. Zhang et al.

Summary: The proposed method combines RT-RPA with CRISPR-Cas12a colorimetric assay for SARS-CoV-2 detection, utilizing DNA-modified gold nanoparticles to achieve high sensitivity and specificity. This novel approach can effectively avoid false positive results from other beta coronavirus members, improving the practical testing availability of SARS-CoV-2.

ANALYTICAL CHEMISTRY (2021)

Article Biochemical Research Methods

Isothermal recombinase polymerase amplification-lateral flow detection of SARS-CoV-2, the etiological agent of COVID-19

Thomas R. Shelite et al.

Summary: The rapid development of an RPA-LF testing method has shown successful detection of SARS-CoV-2, with high accuracy and potential for use in resource-limited and decentralized laboratories.

JOURNAL OF VIROLOGICAL METHODS (2021)

Review Pathology

A review on current diagnostic techniques for COVID-19

Islam El Jaddaoui et al.

Summary: In this review, various diagnostic tests for SARS-CoV-2 are discussed, including molecular techniques targeting viral load, serological methods for antibody detection, and faster assays for detecting the virus in biological samples. The widespread pandemic highlights the importance of mass diagnosis using different techniques for monitoring and containing the spread of COVID-19.

EXPERT REVIEW OF MOLECULAR DIAGNOSTICS (2021)

Review Pathology

Diagnosis for COVID-19: current status and future prospects

M. D. Alamgir Kabir et al.

Summary: This review discusses the origin of the COVID-19 pandemic, detection methods, and market analysis, emphasizing the importance of developing more effective diagnostic devices and urging testing laboratories to invest in multiplexed and scalable detection tools.

EXPERT REVIEW OF MOLECULAR DIAGNOSTICS (2021)

Article Multidisciplinary Sciences

Rapid electrochemical detection of coronavirus SARS-CoV-2

Thanyarat Chaibun et al.

Summary: The researchers developed an ultrasensitive electrochemical biosensor based on isothermal rolling circle amplification for rapid detection of SARS-CoV-2, capable of detecting as low as 1 copy/mu L of N and S genes in clinical samples. The sensor showed a 100% concordance with qRT-PCR results in clinical sample evaluation.

NATURE COMMUNICATIONS (2021)

Editorial Material Medicine, General & Internal

Covid-19: How the UK is using lateral flow tests in the pandemic

Ingrid Torjesen

BMJ-BRITISH MEDICAL JOURNAL (2021)

Article Multidisciplinary Sciences

Expression and clinical significance of IL7R, NFATc2, and RNF213 in familial and sporadic multiple sclerosis

Seyedeh Zahra Hosseini Imani et al.

Summary: Multiple sclerosis (MS) is a chronic inflammatory and autoimmune disorder of the central nervous system. The study found decreased expression of IL7R in sporadic MS patients and increased expression of NFATc2 in MS patients. There was a significant increase in the expression level of the RNF213 gene in familial MS patients.

SCIENTIFIC REPORTS (2021)

Article Multidisciplinary Sciences

Gene expression profiles of YAP1, TAZ, CRB3, and VDR in familial and sporadic multiple sclerosis among an Iranian population

Sheyda Khalilian et al.

Summary: The study found that the Hippo pathway effectors and VDR gene may play a significant role in the pathophysiology of multiple sclerosis, with different gene expression patterns in sporadic and familial forms of the disease, which could have important implications for personalized treatment approaches.

SCIENTIFIC REPORTS (2021)

Article Medical Laboratory Technology

The Hologic Aptima SARS-CoV-2 assay enables high ratio pooling saving reagents and improving turnaround time

Kimberly Newsom et al.

Summary: The Hologic Aptima (TM) TMA SARS-CoV-2 assay was used to test pooled nasopharyngeal samples, showing comparable performance with CDC RT-PCR and Cepheid SARS-CoV-2 assays. Pooled testing can effectively screen for SARS-CoV-2, but attention should be paid to weak-positive samples to avoid reporting false negatives.

JOURNAL OF CLINICAL LABORATORY ANALYSIS (2021)

Article Biochemical Research Methods

Evaluation of the analytical performance and specificity of a SARS-CoV-2 transcription-mediated amplification assay

Markus Schneider et al.

Summary: The study evaluated the analytical performance of Hologic Aptima TMA assay for SARS-CoV-2 RNA detection, showing high sensitivity and specificity, indicating potential feasibility for COVID-19 screening.

JOURNAL OF VIROLOGICAL METHODS (2021)

Article Immunology

A Ligation/Recombinase Polymerase Amplification Assay for Rapid Detection of SARS-CoV-2

Pei Wang et al.

Summary: In this study, a ligation and recombinase polymerase amplification (L/RPA) combined assay was established for rapid detection of SARS-CoV-2, addressing the low efficiency problem on RNA templates and achieving a sensitivity comparable to RT-qPCR.

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY (2021)

Article Engineering, Biomedical

Navigating the Pandemic Response Life Cycle: Molecular Diagnostics and Immunoassays in the Context of COVID-19 Management

Baback Gharizadeh et al.

Summary: This review discusses the clinical insights regarding the use of molecular tests and immunoassays in different phases of COVID-19 management, summarizes the spatial and temporal distribution of viral RNA, antigens, and antibodies during human infection, and shares the lessons learned and obstacles encountered in real world high-volume screening programs.

IEEE REVIEWS IN BIOMEDICAL ENGINEERING (2021)

Article Biochemical Research Methods

A microfluidic-integrated lateral flow recombinase polymerase amplification (MI-IF-RPA) assay for rapid COVID-19 detection

Dan Liu et al.

Summary: The MI-IF-RPA assay developed in this study offers a rapid and sensitive detection of SARS-CoV-2, integrating RT-RPA and LF detection systems into a single microfluidic chip. The assay provides easily interpretable results with a short testing time of approximately 30 minutes, showing high sensitivity and specificity, making it a cost-effective screening tool for resource-limited areas in complement to RT-PCR.

LAB ON A CHIP (2021)

Article Chemistry, Analytical

Two stage, nested isothermal amplification in a single tube

Mohamed El-Tholoth et al.

Summary: Penn-RAMP is a highly sensitive molecular test combining RPA and LAMP techniques, showing superior detection efficiency compared to traditional methods and reducing the likelihood of false negative results.

ANALYST (2021)

Review Chemistry, Analytical

Current advances in the detection of COVID-19 and evaluation of the humoral response

Maria Ana Cristina Huergo et al.

Summary: The new outbreak of COVID-19 caused by coronavirus SARS-CoV-2 in late 2019 has led to the development and re-adaptation of various diagnostic approaches. These approaches include highly sensitive RT-qPCR as the gold standard method, easy POC tests for mass screening, and serology assays for comprehensive detection and surveillance. More controlled serology cohort studies are needed to better understand the immune response to SARS-CoV-2.

ANALYST (2021)

Article Medicine, General & Internal

Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China

Chaolin Huang et al.

LANCET (2020)

Article Multidisciplinary Sciences

A new coronavirus associated with human respiratory disease in China

Fan Wu et al.

NATURE (2020)

Review Pharmacology & Pharmacy

Recent advances and perspectives of nucleic acid detection for coronavirus

Minzhe Shen et al.

JOURNAL OF PHARMACEUTICAL ANALYSIS (2020)

Review Chemistry, Multidisciplinary

Diagnosing COVID-19: The Disease and Tools for Detection

Buddhisha Udugama et al.

ACS NANO (2020)

Review Medical Laboratory Technology

Molecular, serological, and biochemical diagnosis and monitoring of COVID-19: IFCC taskforce evaluation of the latest evidence

Mary Kathryn Bohn et al.

CLINICAL CHEMISTRY AND LABORATORY MEDICINE (2020)

Editorial Material Medicine, General & Internal

Interpreting Diagnostic Tests for SARS-CoV-2

Nandini Sethuraman et al.

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION (2020)

Letter Cell Biology

SARS-CoV-2 detection with CRISPR diagnostics

Lu Guo et al.

CELL DISCOVERY (2020)

Article Biotechnology & Applied Microbiology

RT-LAMP for rapid diagnosis of coronavirus SARS-CoV-2

Wei E. Huang et al.

MICROBIAL BIOTECHNOLOGY (2020)

Editorial Material Medicine, General & Internal

Point-of-Care RNA-Based Diagnostic Device for COVID-19

Ting Yang et al.

DIAGNOSTICS (2020)

Article Infectious Diseases

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2): a global pandemic and treatment strategies

Atul Sharma et al.

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS (2020)

Article Microbiology

Analytical and Clinical Comparison of Three Nucleic Acid Amplification Tests for SARS-CoV-2 Detection

Elizabeth Smith et al.

JOURNAL OF CLINICAL MICROBIOLOGY (2020)

Article Cell Biology

A colorimetric RT-LAMP assay and LAMP-sequencing for detecting SARS-CoV-2 RNA in clinical samples

Viet Loan Dao Thi et al.

SCIENCE TRANSLATIONAL MEDICINE (2020)

News Item Multidisciplinary Sciences

FAST CORONAVIRUS TESTS ARE COMING

Giorgia Guglielmi

NATURE (2020)

Article Multidisciplinary Sciences

Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay

Xiong Ding et al.

NATURE COMMUNICATIONS (2020)

Article Microbiology

Development and evaluation of a rapid CRISPR-based diagnostic for COVID-19

Tieying Hou et al.

PLOS PATHOGENS (2020)

Review Chemistry, Analytical

Diagnostic techniques for COVID-19 and new developments

Elham Sheikhzadeh et al.

TALANTA (2020)

Review Engineering, Biomedical

Evaluation of current diagnostic methods for COVID-19

Saadet Alpdagtas et al.

APL BIOENGINEERING (2020)

Review Immunology

An Update on Molecular Diagnostics for COVID-19

Khursheed Ul Islam et al.

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY (2020)

Review Biotechnology & Applied Microbiology

Mini review: Recent progress in RT-LAMP enabled COVID-19 detection

Dorian Thompson et al.

SENSORS AND ACTUATORS REPORTS (2020)

Article Immunology

CRISPR-based rapid and ultra-sensitive diagnostic test for Mycobacterium tuberculosis

Jing-Wen Ai et al.

EMERGING MICROBES & INFECTIONS (2019)

Article Chemistry, Analytical

Technical aspects of nicking enzyme assisted amplification

Liu Wang et al.

ANALYST (2018)

Article Biochemical Research Methods

Multiplexed isothermal nucleic acid amplification

Olena Mayboroda et al.

ANALYTICAL BIOCHEMISTRY (2018)

Review Chemistry, Analytical

Recombinase polymerase amplification: Basics, applications and recent advances

Ivan Magrina Lobato et al.

TRAC-TRENDS IN ANALYTICAL CHEMISTRY (2018)

Article Microbiology

A Rapid and Specific Assay for the Detection of MERS-CoV

Pei Huang et al.

FRONTIERS IN MICROBIOLOGY (2018)

Letter Cell Biology

CRISPR-Cas12a-assisted nucleic acid detection

Shi-Yuan Li et al.

CELL DISCOVERY (2018)

Review Chemistry, Medicinal

Research Progress on Rolling Circle Amplification (RCA)-Based Biomedical Sensing

Lide Gu et al.

PHARMACEUTICALS (2018)

Article Medical Laboratory Technology

Two-Stage Isothermal Enzymatic Amplification for Concurrent Multiplex Molecular Detection

Jinzhao Song et al.

CLINICAL CHEMISTRY (2017)

Article Chemistry, Analytical

Multiplexed Recombinase Polymerase Amplification Assay To Detect Intestinal Protozoa

Zachary Crannell et al.

ANALYTICAL CHEMISTRY (2016)

Article Chemistry, Analytical

Nicking endonuclease-mediated isothermal exponential amplification for double-stranded DNA detection

Chao Shi et al.

SENSORS AND ACTUATORS B-CHEMICAL (2016)

Review Microbiology

Loop-mediated isothermal amplification (LAMP): principle, features, and future prospects

Tsugunori Notomi et al.

JOURNAL OF MICROBIOLOGY (2015)

Review Pathology

Molecular isothermal techniques for combating infectious diseases: towards low-cost point-of-care diagnostics

Hector David de Paz et al.

EXPERT REVIEW OF MOLECULAR DIAGNOSTICS (2014)

Review Gastroenterology & Hepatology

Recombinase polymerase amplification as a promising tool in hepatitis C virus diagnosis

Hosam Zaghloul et al.

WORLD JOURNAL OF HEPATOLOGY (2014)

Article Biochemical Research Methods

Cas9 as a versatile tool for engineering biology

Prashant Mali et al.

NATURE METHODS (2013)

Article Biochemistry & Molecular Biology

CRISPR-Cas: Evolution of an RNA-based adaptive immunity system in prokaryotes

Eugene V. Koonin et al.

RNA BIOLOGY (2013)

Article Multidisciplinary Sciences

CRISPR provides acquired resistance against viruses in prokaryotes

Rodolphe Barrangou et al.

SCIENCE (2007)

Article Biochemistry & Molecular Biology

DNA detection using recombination proteins

Olaf Piepenburg et al.

PLOS BIOLOGY (2006)

Article Biotechnology & Applied Microbiology

An RNA transcription-based amplification technique (NASBA) for the detection of viable Salmonella enterica

SA Simpkins et al.

LETTERS IN APPLIED MICROBIOLOGY (2000)