期刊
ENVIRONMENTAL RESEARCH
卷 214, 期 -, 页码 -出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.envres.2022.113947
关键词
Aureobasidium pullulans; Pullulan; Melanin; Potato starch industry; Resource utilization
资金
- Heilongjiang Touyan Team [HITTY-20190034]
In this study, the strain Aureobasidium pullulans HIT-LCY3(T) was used to ferment potato starch waste to produce pullulan and melanin. The optimization of culture conditions led to high yields, and the use of adaptive evolution and air-floating fermenters further increased production. Small-scale and pilot tests confirmed the industrial potential of the process.
Pullulan and melanin have become important secondary metabolites that are now widely studied. In this study, a strain of Aureobasidium pullulans HIT-LCY3(T) was used to ferment potato starch industrial waste to produce pullulan and melanin. After optimization, the culture conditions for the fermentation medium were obtained: inoculum age of 48 h, initial pH of 6.0, inoculation quantity of 1.5%, temperature of 26 ?, fermentation time of 5 d and speed of 160 rpm. Under these conditions, the yield of pullulan was 23.47 g/L with a molecular weight (MW) of 1.21 x 10(6) Da and the yield of melanin was 18.98 g/L. In addition, the adaptive evolution could significantly increase the yield of pullulan and melanin and the air-floating fermenters was more conductive to product accumulation. Through the 5 L small-scale test and 1000 L pilot test, the yield of pullulan reached 16.52 g/L with molecular weight of 0.92 x 10(6) Da and the yield of melanin was 12.08 g/L at the trial production of 30,000 L. This work could provide strong support for industrial production and new guidance for waste utilization and environmental protection.
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