期刊
ENVIRONMENTAL RESEARCH
卷 214, 期 -, 页码 -出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.envres.2022.113834
关键词
Heterotrophic nitrification -aerobic denitrification; Salinity inhibition; Transcriptome; Denitrification genes response; Oxidative stress
资金
- Chongqing Technology Innovation and Application Development Special Project, China [cstc2021jszx-cylhx0013]
- Chongqing Technology Innovation and Application Demonstration Project, China [cstc2019jscx- msxmX0418, cstc2020jscx-msxmX0040]
- Scientific Research Foundation of Chongqing University of Technology, China [2019ZD72]
- Science and Technology Planning Project of Chongqing Banan District, China [2020QC380]
- Chongqing Postgraduate Research and Innovation Project, China [CYS21456]
The inhibitory effects and mechanisms of different salt ions on heterotrophic nitrification-aerobic denitrification (HN-AD) bacteria Acinetobacter sp. TAC-1 were studied. NaCl, KCl, and Na2SO4 exerted different levels of inhibition on TAC-1, with Na2SO4 significantly downregulating denitrification gene transcripts.
One of the biggest challenges of applying heterotrophic nitrification-aerobic denitrification (HN-AD) bacteria to treat high salt organic wastewater lies in the inhibitory effect exerted by salinity. To study the inhibition effect and underlying mechanism induced by different ion types and ion composition, the individual and combined effects of NaCl, KCl and Na2SO4 on HN-AD bacteria Acinetobacter sp. TAC-1 were systematically investigated by batch experiments. Results indicated that the ammonia nitrogen removal yield and TAC-1 activity decreased with increased salt concentration. NaCl, KCl and Na2SO4 exerted different degrees of inhibition on TAC-1, with half concentration inhibition constant values of 0.205, 0.238 and 0.110 M, respectively. A synergistic effect on TAC-1 was found with the combinations of NaCl + KCl, NaCl + Na(2)SO(4 )and NaCl + KCl + Na2SO4. The whole RNA resequencing suggested that transcripts of denitrification genes (nirB and nasA) were significantly downregulated with increased Na2SO4 concentration. Simultaneously, Na2SO4 stress disrupted cell respiration, DNA replication, transcription, translation, and induced oxidative stress. Finally, we proposed a conceptual model to summarize the inhibition mechanisms and possible response strategies of TAC-1 bacteria under Na2SO4 stress.
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