Formation mechanism and environmental drivers of Alexandrium catenella bloom events in the coastal waters of Qinhuangdao, China

In the last 5 years, paralytic shellfish toxins (PSTs) have been recurrently detected in mollusks farmed in the mussel culture area of Qinhuangdao city, along with the occurrence of toxic outbreaks linked to dinoflagellate species of the Alexandrium genus. To understand the formation mechanism and variation of these events, continuous and comprehensive PSTs monitoring was carried out between 2017 and 2020. Through the analysis of both phytoplankton and cysts via light microscopy and quantitative polymerase chain reaction, it was shown that Alexandrium catenella was responsible for the production of PSTs, which consisted mainly of gonyautoxins 1,4 (GTX1/4, 87%) and GTX2/3 (13%). During bloom events in 2019, mussels accumulated the highest PSTs value (929 mu g STX di-HCl eq center dot kg 1) in conjunction with the peak of cell abundances, and toxin profiles were consistent with high distributions of GTX1/4, GTX2/3, and Neosaxitoxin. Toxin metabolites vary in different substances and mainly transferred to a stable proportion of a-epimer: ss-epimers 3:1. The environmental drivers of Alexandrium blooms included the continuous rise of water temperature (>4.C) and calm weather with low wind speed and no significant precipitation. By comparing toxin profiles and method sensitivity, it was found that dissolved toxins in seawater are more useful for early warning. These results have important implications for the effective monitoring and management of paralytic shellfish poisoning outbreaks.

Automated summary

In the last 5 years, paralytic shellfish toxins (PSTs) have been detected in mollusks farmed in the mussel culture area of Qinhuangdao city, along with toxic outbreaks linked to Alexandrium dinoflagellate species. Continuous monitoring revealed that Alexandrium catenella was responsible for producing PSTs, mainly GTX1/4 and GTX2/3. Environmental factors such as water temperature and weather conditions played a role in the occurrence of Alexandrium blooms. Dissolved toxins in seawater were found to be more useful for early warning. These findings have important implications for the monitoring and management of paralytic shellfish poisoning outbreaks.