Drought, heat, and their combination impact the root exudation patterns and rhizosphere microbiome in maize roots

Root exudates play an essential role in helping plants to cope with abiotic stress. However, the modulation of root exudation profiles under multiple stress conditions is still poorly understood. Using targeted and untargeted metabolomics, here we tested the effect of drought, heat stress, and their combination on maize root exudates, also considering the differences that might exist between root types (seminal and primary) and root zones (apical and sub-apical). In addition, we built an analytical framework that relates the root exudation profile with the rhizosphere bacterial community, enabling us to dissect the interactions between specific root exudates and bacterial taxa. The composition of root exudates undergoes distinct modulation according to the single or combined stress and to the root zone, but not according to the root type. In addition, we found that stress-specific exudates can influence the relative abundance of specific bacterial taxa, some of which are known to be bene-ficial microorganisms. Our results contribute to the understanding of plant-soil interactions under the influence of abiotic stressors, which is key in paving the way towards an increased resilience to abiotic stresses, repre-senting a powerful tool to craft the next generation of agricultural practices.

Automated summary

Root exudates play a crucial role in helping plants cope with abiotic stress. This study investigated the modulation of root exudation profiles under drought, heat stress, and their combination, as well as the differences in root types and zones. The results showed that the composition of root exudates is significantly modulated by the presence of stress and the root zone, but not by the root type. Additionally, stress-specific exudates were found to influence the abundance of specific beneficial bacterial taxa. This research contributes to understanding plant-soil interactions under abiotic stress and has important implications for developing resilient agricultural practices.