期刊
EMBO REPORTS
卷 23, 期 11, 页码 -出版社
WILEY
DOI: 10.15252/embr.202154507
关键词
Ca2+-dependent secretion; Ca2+-independent but voltage-dependent secretion; dorsal horn; dorsal root ganglion; synaptic transmission
资金
- National Natural Science Foundation of China [31930061, 21790394, 31761133016, 31821091, 31330024, 31171026, 31327901, 32171233, 31670843, 21790390]
- National Key Research and Development Program of China [2016YFA0500401]
- Shaanxi Natural Science Funds for Distinguished Young Scholars of China [2019JC-07]
- Innovation Capability Support Program of Shaanxi Province, China [2021TD-37]
- China Postdoctoral Science Foundation [2020M680211, 2021T140014]
The newly discovered Ca2+-independent but voltage-dependent secretion mechanism plays an important role in central synaptic transmission and can trigger neurotransmitter release through action potentials, with faster vesicle recycling and less short-term depression.
A central principle of synaptic transmission is that action potential-induced presynaptic neurotransmitter release occurs exclusively via Ca2+-dependent secretion (CDS). The discovery and mechanistic investigations of Ca2+-independent but voltage-dependent secretion (CiVDS) have demonstrated that the action potential per se is sufficient to trigger neurotransmission in the somata of primary sensory and sympathetic neurons in mammals. One key question remains, however, whether CiVDS contributes to central synaptic transmission. Here, we report, in the central transmission from presynaptic (dorsal root ganglion) to postsynaptic (spinal dorsal horn) neurons in vitro, (i) excitatory postsynaptic currents (EPSCs) are mediated by glutamate transmission through both CiVDS (up to 87%) and CDS; (ii) CiVDS-mediated EPSCs are independent of extracellular and intracellular Ca2+; (iii) CiVDS is faster than CDS in vesicle recycling with much less short-term depression; (iv) the fusion machinery of CiVDS includes Cav2.2 (voltage sensor) and SNARE (fusion pore). Together, an essential component of activity-induced EPSCs is mediated by CiVDS in a central synapse.
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