期刊
EMBO JOURNAL
卷 41, 期 18, 页码 -出版社
WILEY
DOI: 10.15252/embj.2021110242
关键词
AMPA receptor; associative learning; interactome; proximity labelling; tau
资金
- National Health and Medical Research Council (NHMRC) [1143978, 1176628]
- Australian Research Council (ARC) [DP170100843, DP200102396, DP220101900]
- Dementia Australia Research Foundation
- Flinders Foundation
- Flinders University
- BrightFocus Foundation [A2022022F]
- NHMRC [APP200660]
- ARC [DP180101473]
- Australian Research Council [DP200102396] Funding Source: Australian Research Council
Microtubule-associated protein tau plays a central role in Alzheimer's disease and other tauopathies. This study used proximity-labelling proteomics to examine tau interactions in neurons and mouse brains. The results show that tau interacts with proteins involved in cytoskeletal regulation, synaptic vesicle regulation, and postsynaptic receptor regulation. These interactions are related to Parkinson's disease, Alzheimer's disease, and prion disease. Furthermore, the study demonstrates that tau interacts with N-ethylmaleimide sensitive fusion protein (NSF) and reduces its activity, leading to altered trafficking of AMPA-type glutamate receptors (AMPAR) and enhanced memory formation.
Microtubule-associated protein tau is a central factor in Alzheimer's disease and other tauopathies. However, the physiological functions of tau are unclear. Here, we used proximity-labelling proteomics to chart tau interactomes in primary neurons and mouse brains in vivo. Tau interactors map onto pathways of cytoskeletal, synaptic vesicle and postsynaptic receptor regulation and show significant enrichment for Parkinson's, Alzheimer's and prion disease. We find that tau interacts with and dose-dependently reduces the activity of N-ethylmaleimide sensitive fusion protein (NSF), a vesicular ATPase essential for AMPA-type glutamate receptor (AMPAR) trafficking. Tau-deficient (tau(-/-)) neurons showed mislocalised expression of NSF and enhanced synaptic AMPAR surface levels, reversible through the expression of human tau or inhibition of NSF. Consequently, enhanced AMPAR-mediated associative and object recognition memory in tau(-/-) mice is suppressed by both hippocampal tau and infusion with an NSF-inhibiting peptide. Pathologic mutant tau from mouse models or Alzheimer's disease significantly enhances NSF inhibition. Our results map neuronal tau interactomes and delineate a functional link of tau with NSF in plasticity-associated AMPAR-trafficking and memory.
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