期刊
EMBO JOURNAL
卷 41, 期 21, 页码 -出版社
WILEY
DOI: 10.15252/embj.2022112107
关键词
centrioles; centrosome; ciliogenesis; expansion microscopy; human cells
资金
- Fondation ARC pour la Recherche sur le Cancer [PJA 20151203291, PJA3 2020060002055]
- Schweizerischer Nationalfonds zur Forderung der Wissenschaftlichen Forschung (SNSF) [PP00P3_187198, 310030_205087, IZSEZ0_203806]
- European Research Council ERC ACCENT [StG 715289]
- Universite Paris-Sud
- Fondation ARC pour la Recherche sur le Cancer
- Labex CelTysPhyBio
- EMBO fellowship [ALTF 284-2019]
- Novartis Foundation [18B112]
- Universite de Geneve
- Swiss National Science Foundation (SNF) [310030_205087, IZSEZ0_203806] Funding Source: Swiss National Science Foundation (SNF)
This study demonstrates the presence of a conserved SFI1/Centrin module that displays divergent functions in different organisms.
Over the course of evolution, the centrosome function has been conserved in most eukaryotes, but its core architecture has evolved differently in some clades, with the presence of centrioles in humans and a spindle pole body (SPB) in yeast. Similarly, the composition of these two core elements has diverged, with the exception of Centrin and SFI1, which form a complex in yeast to initiate SPB duplication. However, it remains unclear whether this complex exists at centrioles and whether its function has been conserved. Here, using expansion microscopy, we demonstrate that human SFI1 is a centriolar protein that associates with a pool of Centrin at the distal end of the centriole. We also find that both proteins are recruited early during procentriole assembly and that depletion of SFI1 results in the loss of the distal pool of Centrin, without altering centriole duplication. Instead, we show that SFI1/Centrin complex is essential for centriolar architecture, CEP164 distribution, and CP110 removal during ciliogenesis. Together, our work reveals a conserved SFI1/Centrin module displaying divergent functions between mammals and yeast.
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