Highly selective simultaneous determination of isoniazid and acetaminophen using black phosphorus nanosheets electrochemical sensor

Only few electrochemical sensors had been reported for simultaneous determination of isoniazid (INH) and acetaminophen (APAP). These sensors are limited by simultaneous determination of high concentration INH and APAP at high oxidation potentials, and the detection limits are high, which cannot meet the need for simultaneous determination of INH and APAP at low concentrations in real clinical samples. In this work, a unique peak potential position reversal phenomenon was observed on a black phosphorus nanosheets electrochemical sensor (BP NSs/GCE), which improve the detection selectivity significantly. At low oxidation potential, the BP NSs/GCE sensor realizes the simultaneous determination of INH and APAP in low concentration range, obtained wide linear detection range of INH (0.05 - 3 mu M) and APAP (0.01 - 2.4 mu M), and obtained low detection limit of INH (15.6 nM) and APAP (3.6 nM). Finally, the BP NSs/GCE sensor was successfully applied in the simultaneous determination of INH and APAP in human blood, urine and saliva samples, which is of great significance for the early prevention of liver failure disease in clinical diagnosis.

Automated summary

A unique peak potential position reversal phenomenon was observed on a black phosphorus nanosheets electrochemical sensor, which significantly improved the detection selectivity for simultaneous determination of isoniazid and acetaminophen. The sensor successfully achieved simultaneous determination of the two compounds in low concentration range, with wide linear detection range and low detection limits, and was applied in real clinical samples for early prevention of liver failure disease.