期刊
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
卷 243, 期 -, 页码 -出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2022.114007
关键词
Developmental malformation; Neuroactive ligand-receptor; Neuronal development; Locomotor activity; Dichlorobenzoquinone
资金
- National Key R & D Program of China [2018YFC1004300, 2018YFC1004304]
The disinfection by-product 2, 5-dichloro-1, 4-benuinone (2, 5-DCBQ) exhibits neurodevelopmental toxicity in zebrafish embryos. Exposure to 2, 5-DCBQ resulted in developmental defects and abnormal locomotor activity in zebrafish larvae, with downregulation of genes associated with neuronal development and disruption of neuroactive ligand-receptor interaction and apoptosis pathways.
2, 5-dichloro-1, 4-benuinone (2, 5-DCBQ) is an emerging disinfection by-product belonging to the class of hal-obenzoquinones (HBQs). However, there is limited evidence regarding the neurotoxic effects of 2, 5-DCBQ. To better understand the toxicological mechanisms of aquatic organisms, zebrafish embryos were exposed to 0.2 mg/L, 0.4 mg/L, and 0.6 mg/L of 2, 5-DCBQ from 4 h post-fertilization (hpf) to 120 hpf. Developmental defects, such as reduced body length, decreased heart rate, decreased pigmentation, and abnormal motor axon structure was observed. In particular, the locomotor activity of zebrafish larvae reduced with exposure to increasing 2, 5-DCBQ concentrations, and this effect was more pronounced under dark stimulation. The results indicated that the genes associated with neuronal development (gfap, mbp, syn2a, elavl3, ache, and a1-tubulin) were significantly downregulated after treatment with 2, 5-DCBQ. Furthermore, the KEGG result showed the neuroactive ligand-receptor interaction and apoptosis pathways were visibly disrupted, and we found acetylcholinesterase activ-ity was also affected. In summary, the disinfection by-product, 2, 5-DCBQ, exhibits neurodevelopmental toxicity in zebrafish embryos, providing novel evidence for comprehensive analyses of its toxicity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据