期刊
DEVELOPMENT
卷 149, 期 21, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.201012
关键词
Computing modelling; Condensation step; Kidney development; Nephron progenitor cells
资金
- Academy of Finland [206038, 121647, 250900, 251314, 260056, 243014583]
- Tekes BioRealHealth [24302443]
- Svenska Kulturfonden
- Suomen Kulttuurirahasto (Pekka ja Jukka-Pekka Lylykarin rahasto)
- Schweizerischer Nationalfonds zur Forderung der Wissenschaftlichen Forschung [CRSII5 170930]
- Swiss National Science Foundation (SNF) [CRSII5_170930] Funding Source: Swiss National Science Foundation (SNF)
FGF8 plays a crucial role in the condensation of nephron progenitor cells towards the epithelial ureteric buds in the kidney development, by exerting a strong chemokinetic effect.
Kidneys develop via iterative branching of the ureteric epithelial tree and subsequent nephrogenesis at the branch points. Nephrons form in the cap mesenchyme as the metanephric mesenchyme (MM) condenses around the epithelial ureteric buds (UBs). Previous work has demonstrated that FGF8 is important for the survival of nephron progenitor cells (NPCs), and early deletion of Fgf8 leads to the cessation of nephron formation, which results in post-natal lethality. We now reveal a previously unreported function of FGF8. By combining transgenic mouse models, quantitative imaging assays and data-driven computational modelling, we show that FGF8 has a strong chemokinetic effect and that this chemokinetic effect is important for the condensation of NPCs to the UB. The computational model shows that the motility must be lower close to the UB to achieve NPC attachment. We conclude that the FGF8 signalling pathway is crucial for the coordination of NPC condensation at the UB. Chemokinetic effects have also been described for other FGFs and may be generally important for the formation of mesenchymal condensates.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据