期刊
DEVELOPMENT
卷 149, 期 21, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.200900
关键词
Zar1l; Zar proteins; Meiosis; Xenopus laevis; Translation; RNP
资金
- Deutsche Forschungsgemeinschaft
- Konstanz Research School Chemical Biology of the Universita?
- [CRC 969]
- [STE 2517/1]
- [STE 2517/5-1]
This study elucidates the role of Zar1l in Xenopus oocytes, showing that it accelerates hormone-induced meiotic resumption. It is found that Zar1l forms a ribonucleoparticle complex with other proteins to regulate the translation of specific mRNAs, controlling key cell cycle regulators during oocyte maturation.
Oocyte maturation and early embryo development occur in vertebrates in the near absence of transcription. Thus, sexual reproduction of vertebrates critically depends on the timely translation of mRNAs already stockpiled in the oocyte. Yet how translational activation of specific mRNAs is temporally coordinated is still incompletely understood. Here, we elucidate the function of Zar1l, a yet uncharacterized member of the Zar RNA-binding protein family, in Xenopus oocytes. Employing TRIM-Away, we demonstrate that loss of Zar1l accelerates hormone-induced meiotic resumption of Xenopus oocytes due to premature accumulation of the M-phase -promoting kinase cMos. We show that Zar1l is a constituent of a large ribonucleoparticle containing the translation repressor 4E-T and the central polyadenylation regulator CPEB1, and that it binds directly to the cMos mRNA. Partial, hormone-induced degradation of Zar1l liberates 4E-T from CPEB1, which weakens translational repression of mRNAs encoding cMos and likely additional M-phase-promoting factors. Thus, our study provides fundamental insights into the mechanisms that ensure temporally regulated translation of key cell cycle regulators during oocyte maturation, which is essential for sexual reproductivity.
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