期刊
DEVELOPMENT
卷 149, 期 22, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.200596
关键词
Anther; Apoplastic barrier; Lignin; Tapetum; Arabidopsis
资金
- FrenchAgence Nationale de la Recherche [ANR-17-CE20-0027]
- Schweizerischer Nationalfonds zur Forderung der WissenschaftlichenForschung (NSF)
- Sofja Kovalevskaja programme by theAlexander von Humboldt-Stiftung
- Max Planck Society
- Ministerio de Ciencia, Innovacion y Universidades
- EuropeanCommission NextGenerationEU
- Agence Nationale de la Recherche (ANR) [ANR-17-CE20-0027] Funding Source: Agence Nationale de la Recherche (ANR)
During flowering plant reproduction, the tapetum and developing pollen grains are symplastically isolated from each other, and from other sporophytic tissues, by a structure called the peritapetal strip. This strip prevents the diffusion of substances from outside the cells. The formation and barrier functions of the peritapetal strip depend on two NADPH oxidases.
During flowering plant reproduction, anthers produce pollen grains, the development of which is supported by the tapetum, a nourishing maternal tissue that also contributes non-cell-autonomously to the pollen wall, the resistant external layer on the pollen surface. How the anther restricts movement of the tapetum-derived pollen wall components, while allowing metabolites such as sugars and amino acids to reach the developing pollen, remains unknown. Here, we show experimentally that in arabidopsis thaliana the tapetum and developing pollen are symplastically isolated from each other, and from other sporophytic tissues, from meiosis onwards. We show that the peritapetal strip, an apoplastic structure, separates the tapetum and the pollen grains from other anther cell layers and can prevent the apoplastic diffusion of fluorescent proteins, again from meiosis onwards. The formation and selective barrier functions of the peritapetal strip require two NADPH oxidases, RBOHE and RBOHC, which play a key role in pollen formation. Our results suggest that, together with symplastic isolation, gating of the apoplast around the tapetum may help generate metabolically distinct anther compartments.
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