期刊
DEVELOPMENT
卷 149, 期 21, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.200819
关键词
KEY WORDS; Ribosome profiling; Translational selectivity; Translation; Transcription; Preimplantation embryo development; Bovine
资金
- National Institutes of Health (NIH) Eunice Kennedy Shriver National Institute of Child Health and Human Development [R01HD102533]
- United States Department of Agriculture (USDA) National Institute of Food and Agriculture [2019-67016-29863]
- Grantova Agentura Ceske Republiky (GACR) [22-27301S]
- National Institutes of Health [R01ES032024]
- University of Florida
By using high-resolution ribosome fractionation and low-input ribosome profiling, we have defined the translational landscapes of bovine oocytes and preimplantation embryos. We discovered four modes of translational selectivity in early embryo development and found a strong translational selection of low-abundance transcripts involved in metabolic pathways and lysosomes. These findings provide important insights into the translational regulation during bovine preimplantation development.
High-resolution ribosome fractionation and low-input ribosome profiling of bovine oocytes and preimplantation embryos has enabled us to define the translational landscapes of early embryo development at an unprecedented level. We analyzed the transcriptome and the polysome-and non-polysome-bound RNA profiles of bovine oocytes (germinal vesicle and metaphase II stages) and early embryos at the two-cell, eight-cell, morula and blastocyst stages, and revealed four modes of translational selectivity: (1) selective translation of non-abundant mRNAs; (2) active, but modest translation of a selection of highly expressed mRNAs; (3) translationally suppressed abundant to moderately abundant mRNAs; and (4) mRNAs associated specifically with monosomes. A strong translational selection of low-abundance transcripts involved in metabolic pathways and lysosomes was found throughout bovine embryonic development. Notably, genes involved in mitochondrial function were prioritized for translation. We found that translation largely reflected transcription in oocytes and two-cell embryos, but observed a marked shift in the translational control in eight-cell embryos that was associated with the main phase of embryonic genome activation. Subsequently, transcription and translation become more synchronized in morulae and blastocysts. Taken together, these data reveal a unique spatiotemporal translational regulation that accompanies bovine preimplantation development.
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