4.5 Article

Spike-heparan sulfate interactions in SARS-CoV-2 infection

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Multidisciplinary Sciences

Genome-wide CRISPR screens identify GATA6 as a proviral host factor for SARS-CoV-2 via modulation of ACE2

Ma'ayan Israeli et al.

Summary: In this study, the authors used CRISPR screens to identify host factors necessary for SARS-CoV-2 infection and found that GATA6 is critical for viral entry. They also demonstrated that pharmacological inhibition of GATA6 can inhibit viral infectivity. This research has important implications for the development of anti-SARS-CoV-2 therapeutic strategies.

NATURE COMMUNICATIONS (2022)

Article Chemistry, Multidisciplinary

Synthetic Heparan Sulfate Mimetic Pixatimod (PG545) Potently Inhibits SARS-CoV-2 by Disrupting the Spike-ACE2 Interaction

Scott E. Guimond et al.

Summary: This study found that the synthetic HS mimetic pixatimod can interact with the receptor binding domain of the SARS-CoV-2 spike protein and directly inhibit its binding to ACE2. The results show that pixatimod can effectively inhibit the infection of multiple clinical isolates of SARS-CoV-2 virus and has broad potency against various variants. In a mouse model, pixatimod also reduces viral titers and weight loss. This study provides strong evidence for the potential use of synthetic HS mimetics as a therapeutic approach for COVID-19.

ACS CENTRAL SCIENCE (2022)

Article Biochemistry & Molecular Biology

Genetic Screens Identify Host Factors for SARS-CoV-2 and Common Cold Coronaviruses

Ruofan Wang et al.

Summary: The study identified common host factors for three coronaviruses and critical host pathways supporting infection by all of them, including phosphatidylinositol phosphate biosynthesis and cholesterol homeostasis. Inhibition of these pathways reduced replication of all three coronaviruses, while the lysosomal protein TMEM106B appeared unique to SARS-CoV-2 infection. These findings provide important insights for understanding the coronavirus life cycle and developing host-directed therapies.
Article Biochemistry & Molecular Biology

Genome-Scale Identification of SARS-CoV-2 and Pan-coronavirus Host Factor Networks

William M. Schneider et al.

Summary: The study revealed the host factors and pathways involved in SARS-CoV-2 and seasonal coronavirus infections, providing a rich resource for developing therapeutic strategies for future coronavirus pandemics.
Article Multidisciplinary Sciences

Host and viral determinants for efficient SARS-CoV-2 infection of the human lung

Hin Chu et al.

Summary: Understanding the factors contributing to efficient SARS-CoV-2 infection in human cells can provide insights into the virus's transmissibility and pathogenesis. The study identifies heparan sulfate as an important attachment factor, while sialic acids on ACE2 may restrict efficient spike/ACE2 interaction in lung tissues. Moreover, the presence of a furin-like cleavage site in SARS-CoV-2 spike is crucial for efficient virus replication in human lungs.

NATURE COMMUNICATIONS (2021)

Article Genetics & Heredity

Genome-wide CRISPR screening identifies TMEM106B as a proviral host factor for SARS-CoV-2

Jim Baggen et al.

Summary: Genome-wide CRISPR screens have revealed that the lysosomal protein TMEM106B is essential for SARS-CoV-2 infection. Overexpression of TMEM106B enhances both SARS-CoV-2 and pseudovirus infection, indicating a potential role in viral entry. Single-cell RNA-sequencing data from COVID-19 patient airway cells shows a correlation between TMEM106B expression and SARS-CoV-2 infection.

NATURE GENETICS (2021)

Article Chemistry, Medicinal

On the Selectivity of Heparan Sulfate Recognition by SARS-CoV-2 Spike Glycoprotein

John E. Chittum et al.

Summary: This study identified specific sequences of heparan sulfate (HS) oligosaccharides that can act as potent antagonists against SARS-CoV-2, offering potential for developing inhibitors of early viral entry steps. Competitive and virtual screening studies revealed the importance of certain HS structures for selective recognition of the virus receptor-binding domain, suggesting a novel pharmacophore for engineered selectivity. This work highlights the key structural motif in HS that could lead to effective and selective agents against SARS-CoV-2.

ACS MEDICINAL CHEMISTRY LETTERS (2021)

Article Biophysics

The flexibility of ACE2 in the context of SARS-CoV-2 infection

Emilia P. Barros et al.

Summary: The COVID-19 pandemic has caused significant loss of life worldwide, and there are still unanswered questions regarding the viral association to ACE2 receptor. Molecular dynamics simulations reveal the flexibility of the ACE2 receptor and its dynamics on the cell surface, which could provide insights for the development of therapeutics against SARS-CoV-2.

BIOPHYSICAL JOURNAL (2021)

Letter Cell Biology

SARS-CoV-2 cell tropism and multiorgan infection

Jia Liu et al.

CELL DISCOVERY (2021)

Article Biochemistry & Molecular Biology

Infection and transmission of SARS-CoV-2 depend on heparan sulfate proteoglycans

Marta Bermejo-Jambrina et al.

Summary: The study identified heparan sulfate proteoglycans as attachment receptors for SARS-CoV-2, with neutralizing antibodies potentially neutralizing infection by interfering with virus binding. Low molecular weight heparins were found to block virus binding to epithelial cells.

EMBO JOURNAL (2021)

Article Chemistry, Multidisciplinary

A glycan gate controls opening of the SARS-CoV-2 spike protein

Terra Sztain et al.

Summary: Through simulations, the study reveals the mechanism of spike protein receptor binding domain (RBD) opening in SARS-CoV-2 infection, highlighting the crucial role of N-glycan in facilitating RBD opening. This research represents a milestone in ensemble pathway simulations and provides a foundation for understanding the fundamental mechanisms of viral entry and infection.

NATURE CHEMISTRY (2021)

Article Multidisciplinary Sciences

SARS-CoV-2 immune evasion by the B.1.427/B.1.429 variant of concern

Matthew McCallum et al.

Summary: The novel CAL.20C (B.1.427/B.1.429) variant carries spike protein mutations, resulting in reduced neutralizing titers in vaccinated individuals and convalescent individuals. The L452R mutation reduces neutralizing activity in RBD-specific monoclonal antibodies, while the S13I and W152C mutations lead to the total loss of neutralization in NTD-specific antibodies due to antigenic supersite remodeling.

SCIENCE (2021)

Article Cell Biology

Systematic analysis of SARS-CoV-2 infection of an ACE2-negative human airway cell

Maritza Puray-Chavez et al.

Summary: This study identifies human H522 lung adenocarcinoma cells as permissive to SARS-CoV-2 infection and reveals the importance of the E484D S variant for infection. Vaccinated individuals' sera block the alternative entry mechanism, whereas convalescent sera are less effective.

CELL REPORTS (2021)

Article Chemistry, Multidisciplinary

Heparan Sulfate Proteoglycans as Attachment Factor for SARSCoV-2

Lin Liu et al.

Summary: The study revealed the binding capability of SARS-CoV-2 spike protein with heparan sulfate (HS) and highlighted the potential therapeutic use of HS oligosaccharides. Heparin showed a higher affinity for SARS-CoV-2 spike protein compared to the receptor binding domain (RBD) alone, inhibiting RBD binding to cells. The findings support a model where HS acts as the initial attachment point for the virus to travel through the glycocalyx and engage with ACE2 for cell entry.

ACS CENTRAL SCIENCE (2021)

Article Biochemistry & Molecular Biology

Heparan Sulfate Facilitates Spike Protein-Mediated SARS-CoV-2 Host Cell Invasion and Contributes to Increased Infection of SARS-CoV-2 G614 Mutant and in Lung Cancer

Jingwen Yue et al.

Summary: This study reveals that the binding of heparan sulfate (HS) to SARS-CoV-2 spike protein (SV2-S) is essential for the virus to infect host cells, and can be inhibited by heparin lyase, HS antagonist surfen, heparin, and heparin derivatives. The higher infectivity of the SARS-CoV-2 G614 variant is attributed to its higher affinity to heparin/HS and increased HS expression on host cells, which can be effectively blocked by heparin lyase and heparin.

FRONTIERS IN MOLECULAR BIOSCIENCES (2021)

Article Biochemistry & Molecular Biology

Evidence of a putative glycosaminoglycan binding site on the glycosylated SARS-CoV-2 spike protein N-terminal domain

Zachariah P. Schuurs et al.

Summary: A new GAG binding site was identified in the spike protein, which may be crucial for understanding recent UK and South African strains. These findings could also aid in future targeted therapy programs.

COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL (2021)

Article Virology

Effective Inhibition of SARS-CoV-2 Entry by Heparin and Enoxaparin Derivatives

Ritesh Tandon et al.

Summary: SARS-CoV-2 virus relies on spike glycoprotein for cell entry, and several sulfated polysaccharides have shown potent neutralizing effects against the virus, demonstrating potential prophylactic and therapeutic applications.

JOURNAL OF VIROLOGY (2021)

Review Pharmacology & Pharmacy

Discovering small-molecule therapeutics against SARS-CoV-2

Vaibhav Tiwari et al.

DRUG DISCOVERY TODAY (2020)

Article Biochemistry & Molecular Biology

SARS-CoV-2 Infection Depends on Cellular Heparan Sulfate and ACE2

Thomas Mandel Clausen et al.

Article Multidisciplinary Sciences

Structures and distributions of SARS-CoV-2 spike proteins on intact virions

Zunlong Ke et al.

NATURE (2020)

Article Chemistry, Multidisciplinary

Beyond Shielding: The Roles of Glycans in the SARS-CoV-2 Spike Protein

Lorenzo Casalino et al.

ACS CENTRAL SCIENCE (2020)

Article Biochemistry & Molecular Biology

Updates to the Symbol Nomenclature for Glycans guidelines

Sriram Neelamegham et al.

GLYCOBIOLOGY (2019)

Article Virology

Entry of Human Coronavirus NL63 into the Cell

Aleksandra Milewska et al.

JOURNAL OF VIROLOGY (2018)

Article Multidisciplinary Sciences

Identification of sialic acid-binding function for the Middle East respiratory syndrome coronavirus spike glycoprotein

Wentao Li et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2017)

Article Biochemistry & Molecular Biology

Glycan shield and epitope masking of a coronavirus spike protein observed by cryo-electron microscopy

Alexandra C. Walls et al.

NATURE STRUCTURAL & MOLECULAR BIOLOGY (2016)

Article Biochemistry & Molecular Biology

Glycan shield and epitope masking of a coronavirus spike protein observed by cryo-electron microscopy

Alexandra C. Walls et al.

NATURE STRUCTURAL & MOLECULAR BIOLOGY (2016)

Review Biochemistry & Molecular Biology

Demystifying Heparan Sulfate-Protein Interactions

Ding Xu et al.

ANNUAL REVIEW OF BIOCHEMISTRY, VOL 83 (2014)

Review Microbiology

The sweet spot: defining virus-sialic acid interactions

Jennifer E. Stencel-Baerenwald et al.

NATURE REVIEWS MICROBIOLOGY (2014)

Article Biochemistry & Molecular Biology

Molecular Mediators for Raft-dependent Endocytosis of Syndecan-1, a Highly Conserved, Multifunctional Receptor

Keyang Chen et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2013)

Review Immunology

Human immunodeficiency virus and heparan sulfate: from attachment to entry inhibition

Bridgette J. Connell et al.

FRONTIERS IN IMMUNOLOGY (2013)

Article Biochemistry & Molecular Biology

Novel heparan sulfate structures revealed by monoclonal antibodies

J van den Born et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2005)

Article Physiology

Heparan sulfates expressed in the distal lung are required for Fgf10 binding to the epithelium and for airway branching

KI Izvolsky et al.

AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY (2003)