GSH responsive traditional clinical drugs probe for cancer cell fluorescence imaging and therapy

Despite the rapid development of fluorescence detection modalities for disease diagnosis, novel fluorescent molecules and probes still face with tremendous pressure to transform before employing such fluorescent tools in the clinic. Impressively, the fluorescent probes based on the traditional fluorescent dye are expected to accelerate the transformation process. Herein, methylene blue is requisitioned to design the GSH responsive probe MB-SS-CPT elaborately. The as-synthesized MB-SS-CPT provides a dramatic optical advantage for GSH detection in vitro , cell fluorescence imaging, in vivo imaging, and antitumor therapy. (c) 2023 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.

Automated summary

Despite the rapid development of fluorescence detection modalities for disease diagnosis, there is still immense pressure for novel fluorescent molecules and probes to transform before their clinic application. Remarkably, fluorescent probes based on traditional fluorescent dyes are expected to expedite this transformation process. In this study, methylene blue is utilized to intricately design the GSH responsive probe MB-SS-CPT. The synthesized MB-SS-CPT provides significant optical advantages for GSH detection in vitro, cell fluorescence imaging, in vivo imaging, and antitumor therapy.