Optimization of consolidated bioprocessing by response surface methodology in the conversion of corn stover to bioethanol by thermophilic Geobacillus thermoglucosidasius

The swift depletion of fossil fuels and their associated environment and economic impact has led the world to explore the sustainable alternate fuels. Amidst the available alternatives lignocellulosic bioethanol provides the edge over the exhausting fossil fuels. In this current study, Response surface methodology, a mathematical and statistical tool was used to optimize the fermentation conditions in consolidated bioprocessing of corn stover by Geobacillus thermoglucosidasius. The impact of inoculum concentration, temperature, pH, agitation speed and time in bioethanol fermentation were screened with Plackett-Burman design and it was farther optimized with central composite design. The analysis by PBD confirmed the significant impact of fermentation time, inoculum concentration, and temperature of the fermentation process. Further, it was optimized with CCD. This showed that 15% v/v of Inoculum concentration, 50 degrees C of temperature and fermentation time of 72 h increased the bioethanol concentration to a maximum of 9.04 g/L with 0.45 g/g significant yield and a conversion efficiency of 88%. Thus, the CCD showed a satisfactory result in consolidated bioprocessing of bioethanol from corn stover. Thus, in the future, this approach of optimization will yield a good base for consistent production of bioethanol.

Automated summary

This study optimized the fermentation conditions of Geobacillus thermoglucosidasius for consolidated bioprocessing of corn stover using response surface methodology. The results showed that under specific fermentation conditions, higher bioethanol concentration and yield can be obtained.