Metabolic pathways reveal the effect of fungicide loaded metal-organic frameworks on the growth of wheat seedlings

Metal-organic frameworks (MOF) are an emerging class of hybrid inorganic-organic porous materials used in various fields, especially in molecule delivery system. As iron is an essential micronutrient for plant growth, iron -based MOF (Fe-MOF) is developed for agricultural application as fungicide carriers. However, fungicides may have various effect on the plant growth, which may be different from Fe-MOF. When they are combined with the carriers, the effects on target plants will change. In this work, tebuconazole-loaded Fe-MOF was prepared and used to treat wheat seedlings. The physiological, biochemical and metabolic levels of wheat roots and shoots were shown by a comparative study. Related metabolic pathways were analyzed by non-targeted metabolomic method. Many metabolites in wheat roots and shoots showed an upward trend after Fe-MOF treatment, but tebuconazole had a negative impact on these indicators. Related metabolic pathways in Fe-MOF and tebuco-nazole treatment were different, and the related pathway of tebuconazole-loaded Fe-MOF was closer to that of Fe-MOF. The metabolic pathways study revealed that the negative impact from tebuconazole was mitigated when wheat seedlings were treated with tebuconazole-loaded Fe-MOF. This research firstly explores the mechanism of MOF as carriers to help plant reduce the negative effects from fungicide by regulating metabolic pathways.

Automated summary

This study investigates the effects of tebuconazole and iron-based MOF (Fe-MOF) on the physiological, biochemical, and metabolic levels of wheat seedlings. The results show that tebuconazole has a negative impact on these indicators, but when combined with Fe-MOF, the negative effects are mitigated.