4.7 Article

Gene expression changes in Epinephelus marginatus (Teleostei, Serranidae) liver reveals candidate molecular biomarker of iron ore contamination

期刊

CHEMOSPHERE
卷 303, 期 -, 页码 -

出版社

PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.chemosphere.2022.134899

关键词

Dusky grouper; Ferritin; Epoxide hydrolase; Cytochrome b reductase; Ecotoxicology

资金

  1. Fundacao de Amparo a Pesquisa do Espirito Santo (FAPES) [01/2015, 570/2018]
  2. Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [001]
  3. Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2020/00109-6]
  4. Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)

向作者/读者索取更多资源

Through gene expression analysis, biomarkers of environmental exposure to iron ore in dusky grouper have been identified, with the upregulation of one specific enzyme validated.
Wastes from iron ore mining activities are potentially damaging to adjacent aquatic ecosystems. We aimed to determine biomarkers of environmental exposure to this xenobiotic in the dusky grouper Epinephelus marginatus by differential gene expression analysis. For this, fish were exposed to iron ore (15.2 mg/L) and gene expression in liver was assessed by RNA-Seq and compared to the control group. A total of 124 differentially expressed genes were identified, from which 52 were upregulated and 72 were downregulated in response to iron ore. From these, ferritin (medium subunit), cytochrome b reductase and epoxide hydrolase genes were selected for validation by RT-qPCR that confirmed the upregulation of epoxide hydrolase in fish exposed to iron ore.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据