期刊
CHEMOSPHERE
卷 303, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.chemosphere.2022.134899
关键词
Dusky grouper; Ferritin; Epoxide hydrolase; Cytochrome b reductase; Ecotoxicology
资金
- Fundacao de Amparo a Pesquisa do Espirito Santo (FAPES) [01/2015, 570/2018]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [001]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2020/00109-6]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
Through gene expression analysis, biomarkers of environmental exposure to iron ore in dusky grouper have been identified, with the upregulation of one specific enzyme validated.
Wastes from iron ore mining activities are potentially damaging to adjacent aquatic ecosystems. We aimed to determine biomarkers of environmental exposure to this xenobiotic in the dusky grouper Epinephelus marginatus by differential gene expression analysis. For this, fish were exposed to iron ore (15.2 mg/L) and gene expression in liver was assessed by RNA-Seq and compared to the control group. A total of 124 differentially expressed genes were identified, from which 52 were upregulated and 72 were downregulated in response to iron ore. From these, ferritin (medium subunit), cytochrome b reductase and epoxide hydrolase genes were selected for validation by RT-qPCR that confirmed the upregulation of epoxide hydrolase in fish exposed to iron ore.
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