期刊
CHEMICAL ENGINEERING JOURNAL
卷 445, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.cej.2022.136633
关键词
Enzymatic hydrolysis; Microreactor; Sugar production; Immobilization; Bioenergy
资金
- State Key Program of National Natural Science of China [51836001]
- Creative Research Groups of the National Natural Science Foundation of China [52021004]
- National Natural Science Funds for Excellent Young Scholar [52022015]
In this study, a microreactor loaded with cellulase based on co-deposition of dopamine (DA) and polyethyleneimine (PEI) was proposed to efficiently hydrolyze carboxymethyl cellulose (CMC) and produce sugar. The cellulase-loaded microreactor showed better temperature adaptability and higher long-term stability than other reaction systems, resulting in higher glucose production per unit time.
Enzymatic hydrolysis is considered to be a restricted step for lignocellulosic bioethanol production at an industrial scale for its high production costs and low efficiency. In this study, a microreactor loaded with cellulase based on co-deposition of dopamine (DA) and polyethyleneimine (PEI) was proposed, for the first time, to efficiently hydrolyze carboxymethyl cellulose (CMC) and produce sugar. When the concentration of PEI increased from 0 to 0.5 mg mL-1, the enzyme loading was increased by 27.4%, and glucose production was increased by 33.2%. Besides, the cellulase-loaded microreactor showed better temperature adaptability than free cellulase at elevated temperatures ranging from 55 degrees C to 65 degrees C. After storage for 20 days, the cellulase-loaded microreactor retained 84.4% of its initial activity, which is 69.5% higher than the free cellulase. The yield reached 169.7 mg glucose g-1 CMC in the cellulase-loaded microreactor in 16 h. With the same amount of enzyme input, the glucose production per unit time in the cellulase-loaded microreactor is 97.2% higher than that in other reaction systems. This study demonstrated that continuous production of glucose can be achieved in the microreactor at a relatively low enzyme dosage.
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