Synthetic Approaches to Break the Chemical Shift Degeneracy of Glycans

NMR spectroscopy is the leading technique for determining glycans' three-dimensional structure and dynamic in solution as well as a fundamental tool to study protein-glycan interactions. To overcome the severe chemical shift degeneracy of these compounds, synthetic probes carrying NMR-active nuclei (e.g., C-13 or F-19) or lanthanide tags have been proposed. These elegant strategies permitted to simplify the complex NMR analysis of unlabeled analogues, shining light on glycans' conformational aspects and interaction with proteins. Here, we highlight some key achievements in the synthesis of specifically labeled glycan probes and their contribution towards the fundamental understanding of glycans.

Automated summary

NMR spectroscopy is vital for understanding the structure and dynamics of glycans. Synthetic probes with specific labels have simplified the complex NMR analysis of these compounds, providing insights into glycans' conformational aspects and their interaction with proteins.