期刊
CELLULOSE
卷 29, 期 16, 页码 8509-8520出版社
SPRINGER
DOI: 10.1007/s10570-022-04799-w
关键词
Bacterial cellulose; Komagataeibacter xylinus; Morphology; Characterization; Genetic stability; Quality control
资金
- Natural Science Foundation of Fujian [2021J01503]
A genetically stable bacterial strain FM883 was isolated from kombucha tea, which efficiently produces high-quality bacterial cellulose (BC) with excellent thermostability in modified media. The BC biosynthesized by FM883 demonstrates excellent production quality control, desired for commercial manufacturing.
The quality control of the bacterial cellulose (BC) production has been facing challenges due to the genetic stability of bacterial strains, which is often overlooked or underestimated. Thus, its commercialized application is limited. Therefore, a bacterial strain FM883 with a 16S rRNA sequence 97% identical to Komagataeibacter xylinus NBRC15237(T) was isolated from kombucha tea. Cultivation of FM883 was conducted in series to observe the morphology and BC yield among different generations from 1st generation to 30th generation. The results show that FM883 can efficiently produce BC in modified Hestrin-Schramn media at the 30th generation (163.5 +/- 0.3 g/L), which was even higher than the 1st generation (157.6 +/- 0.3 g/L). This indicated that FM883 has strong genetic stability. Furthermore, several characterizations of the BC indicated that the quality of the BC biosynthesized by FM883 was good with excellent thermostability. With such strong genetic stability, and properties, as well as sufficiently high consistency and high yield, BC biosynthesized by FM883 had demonstrated excellent production quality control, which is desired for commercial manufacturing.
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