Siloxane-modified bacterial cellulose as a promising platform for cell culture

Cellulose is a versatile tunable material that finds application in the biomedical field as substrate for cell culture. However, few studies have been done to explore the use of bacterial cellulose (BC), a naturally occurring nanofibrillar material with distinct properties, as a platform to produce such device. In the present work, BC membranes have been functionalized with thiol functional group (SH) through silanization reaction with (3-mercaptopropyl)trimethoxysilane (MPTMS) under different conditions, in order to obtain a platform with improved cell adhesion. The efficiency of BC surface modification with MPTMS was evaluated by using acid and base catalyzed reactions, and two different drying methods: at room temperature of 28 degrees C and at 120 degrees C as curing temperature. The results of the set of analyses performed-ATR-FTIR, TGA, elemental analysis,C-13 NMR, contact angle and SEM-indicate that BC surface functionalization was efficient, regardless the drying process. The MPTMS-modified platforms exhibited sulfur content of 3-5 times higher than native BC. The performed biological assay with fibroblast GM07492 human cells revealed that adhesion of cells to the BC surface depends not only on the functional group present at the matrix but also on surface wettability.

Automated summary

This study functionalized bacterial cellulose (BC) membranes to improve cell adhesion. The results showed that the MPTMS modification on BC surface was efficient regardless of the drying process. Cell adhesion to the BC surface depends on both the functional group in the matrix and the surface wettability.