期刊
CELLULAR SIGNALLING
卷 99, 期 -, 页码 -出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cellsig.2022.110433
关键词
Isoimperatorin; CXCR4; EMT; Metastasis; NF-?B; MMP-9; 2
类别
资金
- National Research Foundation of Korea (NRF) - Korean Government (MSIP) [NRF-2021R1I1A2060024, NRF-2022R1I1A1A01071593]
This study found that IIPT derived from Angelica dahurica can potentially function as a novel anti-metastatic agent by inhibiting EMT and metastasis process via inhibition of NF-kappa B activation and CXCR4 expression.
Worldwide, metastatic spread of tumor is the major cause of cancer-related deaths. There are many anti -metastatic agents that can target metastasis-related pathways, but there are relatively few studies on agents targeting C-X-C chemokine receptor type 4 (CXCR4) signaling axis. Here, we have investigated whether Iso-imperatorin (IIPT), derived from Angelica dahurica can modulate CXCR4 signaling axis-related epithelial-to-mesenchymal transition (EMT) and tumor metastasis process. We evaluated the influence of IIPT on CXCR4, HER2, MMPs, EMT markers, and NF-kappa B signaling pathway by using Western blot analysis. The cellular invasion and migration were observed by Boyden chamber and wound healing assays. IIPT has a down-regulatory effect on CXCR4, HER2, and MMP-9/2. On the contrary, imperatorin (IPT) as compared to IIPT did not alter the expression of CXCR4. IIPT down-regulated the protein levels and RNA levels of mesenchymal markers, twist, snail, and enhanced the levels of different epithelial markers. IIPT also inhibited cell migration, invasion, and proliferation. Furthermore, IIPT negatively regulated constitutive NF-kappa B activation and inhibited the trans -location of phospho-p65 and p65 into the nuclei. IIPT can potentially function as a novel anti-metastatic agent by inhibiting EMT and metastasis process via inhibition of NF-kappa B activation and CXCR4 expression in colorectal and hepatocellular carcinoma cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据