期刊
CELL BIOLOGY INTERNATIONAL
卷 46, 期 11, 页码 1937-1946出版社
WILEY
DOI: 10.1002/cbin.11889
关键词
FGD5-AS1; miR-154-5p; osteosarcoma; WNT5A
类别
资金
- Natural Science Foundation of Jilin Provincial Department of Science and Technology (Jilin Provincial Key Laboratory Project and Theme Scientist Project) [20190201282JC]
FGD5-AS1 enhances chemotherapy resistance in osteosarcoma by upregulating WNT5A through sponging miR-154-5p, leading to induced cell autophagy.
Osteosarcoma is prevalent in children and adolescent. The oncogenic function of long-chain noncoding RNA (lncRNA) FGD5 antisense RNA 1 (FGD5-AS1) has been reported. However, the function of FGD5-AS1 in doxorubicin-resistance in osteosarcoma remains to be illucidated. Quantitative real-time PCR (qRT-PCR) and western blot analysis (WB) were used to measure the expression of FGD5-AS1, miR-154-5p, WNT5A and autophagy proteins. MTT assay was used to assess cell viability and transwell assay was performed to evaluate migration. A nude mouse xenograft model was developed to verify the function of FGD5-AS1 in vivo. FGD5-AS1 was upregulated in doxorubicin-resistant (DXR) osteosarcoma cells. Knockdown of FGD5-AS1 suppressed osteosarcoma cell proliferation, migration, and autophagy. FGD5-AS1 upregulated WNT5A expression via sponging miR-154-5p. Furthermore, FGD5-AS1 enhanced osteosarcoma cell chemotherapy resistance through upregulation of WNT5A by inhibiting miR-154-5p. Suppression of FGD5-AS1 significantly suppressed tumor growth in nude mice. FGD5-AS1 may promote chemoresistance through WNT5A-induced autophagy by sponging miR-154-5p in osteosarcoma cells.
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