期刊
CELL
卷 185, 期 19, 页码 3520-+出版社
CELL PRESS
DOI: 10.1016/j.cell.2022.07.019
关键词
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资金
- TED Audacious Project
- Eric and Wendy Schmidt
- Bill and Melinda Gates Foundation [INV-010680]
- Open Philanthropy Project Improving Protein Design Fund
- Takeda
- Washington State Supplement Funding
- Department of Defense CDMRP Prostate Cancer Research Program Postdoctoral Training Grant
- Simons Foundation
- Defense Threat Reduction Agency [HDTRA1-19-1-0003]
- National Institutes of Health [R01AG063845, R01GM120574, R35-GM141818]
- NIH [F32GM120791-02]
- Washington Research Foundation
This study investigates the design principles for macrocycle membrane permeability and oral bioavailability using computational design and experimental characterization. The results show that membrane permeability can be achieved by engaging all amide (NH) groups in internal hydrogen bonding interactions. Additionally, designs with exposed NH groups can be made membrane permeable through the design of an alternative isoenergetic fully hydrogen-bonded state favored in the lipid membrane. The ability to design membrane-permeable and orally bioavailable peptides with high structural accuracy has significant implications for the development of next-generation macrocycle therapeutics.
We use computational design coupled with experimental characterization to systematically investigate the design principles for macrocycle membrane permeability and oral bioavailability. We designed 184 6-12 residue macrocycles with a wide range of predicted structures containing noncanonical backbone modifications and experimentally determined structures of 35; 29 are very close to the computational models. With such control, we show that membrane permeability can be systematically achieved by ensuring all amide (NH) groups are engaged in internal hydrogen bonding interactions. 84 designs over the 6-12 residue size range cross membranes with an apparent permeability greater than 1 3 10(-6) cm/s. Designs with exposed NH groups can be made membrane permeable through the design of an alternative isoenergetic fully hydrogen-bonded state favored in the lipid membrane. The ability to robustly design membrane-permeable and orally bioavailable peptides with high structural accuracy should contribute to the next generation of designed macrocycle therapeutics.
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