期刊
BRAZILIAN JOURNAL OF MICROBIOLOGY
卷 53, 期 4, 页码 1951-1958出版社
SPRINGER
DOI: 10.1007/s42770-022-00826-x
关键词
Bacterial meningitis; Real-time PCR; Streptococcus pneumoniae; Cerebrospinal fluid; Neisseria meningitidis; Haemophilus influenzae
类别
资金
- IIR Investigator's Initiative Research from Pfizer [WI183161]
Bacterial meningitis remains a serious public health concern despite the introduction of several vaccines. Rapid, sensitive, and specific laboratory assays are critical for effective diagnosis and treatment, especially in countries with low culture positivity rates. This study evaluated the use of real-time polymerase chain reaction (RT-PCR) as a rapid diagnostic test for bacterial meningitis in Mexico.
Bacterial meningitis is one of the diseases that, despite the introduction of several vaccines, remains a serious public health concern. Streptococcus pneumoniae (Spn), Neisseria meningitidis (Nm), and Haemophilus influenzae (Hi) are responsible for most cases diagnosed in children, adolescents, and adult population. Rapid, sensitive, and specific laboratory assays are critical for effective diagnosis and treatment, particularly in countries like Mexico in which culture positivity rates are very low due to the use of antibiotics prior to sample collection and to delay in transporting samples to the laboratory. The aim of this study was to evaluate the use of real-time polymerase chain reaction (RT-PCR) of cerebrospinal fluid (CSF) as a rapid diagnostic test for bacterial meningitis and compare these results with bacterial culture in three general hospitals in Mexico. During a 5-year period (2014-2018), a total of 512 CSF samples obtained from patients in whom infectious meningitis was suspected as initial clinical diagnosis were tested with RT-PCR with species-specific targets for the three pathogens. For Spn, 5.07% samples were RT-PCR positive; 0.39% for Nm and none for Hi. Only five RT-PCR Spn positive samples had a positive culture. Sensitivity and specificity estimates for RT-PCR are 100% and 95.46%, respectively. DNA amplification methods can provide better sensitive diagnostic tests than the reference standard, which is culture, particularly when antimicrobial treatment is initiated before clinical samples can be obtained.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据