期刊
BRAZILIAN JOURNAL OF MICROBIOLOGY
卷 53, 期 4, 页码 1857-1870出版社
SPRINGER
DOI: 10.1007/s42770-022-00827-w
关键词
Antioxidant activity; Antibacterial activity; Bioactive compounds; Endophytic fungi; Gynura procumbens; Macrophomina phaseolina
类别
资金
- Tun Zaidi Chair Research Grant Scheme (TZC) [F07(ZRC04)/1237/2015(01)]
This research evaluated the bioactivity of twelve endophytic fungi isolated and characterised from Gynura procumbens. The fungal extracts displayed inhibitory activity against various bacteria, as well as high antioxidant activity. Macrophomina phaseolina SN6 ethyl acetate extract showed the strongest antioxidant activity, while Mycoleptodiscus indicus SN4 methanolic extract exhibited the highest antibacterial activity.
This research evaluates the bioactivity of twelve endophytic fungi successfully isolated and characterised from Gynura procumbens. The fungal extracts displayed inhibitory activity against Staphylococcus aureus, Pseudomonas aeruginosa, Methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli and Salmonella typhi with the MIC and MBC of 5000 mu g/mL. High antioxidant activity using DPPH free radical scavenging assay with inhibition of 86.6% and IC50 value of 104.25 +/- 18.51 mu g/mL were exhibited by ethyl acetate extract of Macrophomina phaseolina SN6. In contrast, the highest scavenging activity percentage of methanolic extract was exhibited by Mycoleptodiscus indicus SN4 (50.0%). Besides that, the highest ferric reducing antioxidant power (FRAP) value of ethyl acetate and methanolic extract was recorded from M. phaseolina SN6 (239.9 mg Fe (II)/g) and M. indicus SN4 (44.7 mg Fe (II)/g), respectively. Total phenolic content (TPC) and total flavonoid content (TFC) of ethyl acetate and methanolic fungal extracts were determined using Folin-Ciocalteu and aluminium chloride, respectively. The highest TPC for ethyl acetate and methanolic extracts were exhibited by Colletotrichum gloeosporioides SN11 (87.0 mg GAE/g) and M. indicus SN4 (35.0 mg GAE/g), whereas the highest TFC of ethyl acetate and methanolic extracts were showed by M. phaseolina SN6 (122.8 mg QCE/g) and M. indicus SN4 (60.4 mg QCE/g), respectively. Bioactive metabolites of isoelemicin (50.8%), terpinen-4-ol (21.5%), eucalyptol (24.3%), oleic acid (19.8%) and beta-pinene (10.9%) were detected. Owing to the higher content of phytochemicals represented in the ethyl acetate extract of M. phaseolina, SN6 is therefore identified to be a superior candidate in exhibiting strong antioxidant and antimicrobial properties be fit for further pharmaceutical studies.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据