期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 119, 期 12, 页码 3474-3486出版社
WILEY
DOI: 10.1002/bit.28223
关键词
acetone-butanol-ethanol fermentation; Clostridium acetobutylicum; continuous fermentation; fibrous-bed bioreactor
资金
- National Science Foundation STTR program [IIP-0810568, IIP-1026648]
- Ohio Department of Development-Third Frontier Advanced Energy Program [08-036]
This study achieved continuous production of butanol from glucose and butyrate by immobilizing cells in a single-pass fibrous-bed bioreactor. The high density of immobilized cells improved the tolerance to butanol and butyric acid, resulting in significantly increased productivity.
Biobutanol produced in acetone-butanol-ethanol (ABE) fermentation at batch mode cannot compete with chemically derived butanol because of the low reactor productivity. Continuous fermentation can dramatically enhance productivity and lower capital arid operating costs, but are rarely used in industrial fermentation because of increased risks of culture degeneration, cell washout, and contamination. In this study, cells of the asporogenous Clostridium acetobutylicum ATCC55025 were immobilized in a single-pass fibrous-bed bioreactor (FBB) for continuous production of butanol from glucose and butyrate at various dilution rates. Butyric acid in the feed medium helped maintaining cells in the solventogenic phase for stable continuous butanol production. At a dilution rate of 1.88 h(-1), butanol was produced at 9.55 g/L, with a yield of 0.24 g/g and productivity of 16.8 g/L/h, which was the highest productivity ever achieved for biobutanol fermentation and an 80-fold improvement over the conventional ABE fermentation. The extremely high productivity was attributed to the high density of viable cells (similar to 100 g/L at >70% viability) immobilized in the fibrous matrix, which also enabled the cells to better tolerate butanol and butyric acid. The FBB was stable for continuous operation for an extended period of over 1 month.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据