期刊
BIOSENSORS & BIOELECTRONICS
卷 215, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2022.114563
关键词
Silica nanochannel array; Bipolar surface charges; Solid-state electrochemiluminescence; Immunosensor; SARS-CoV-2 IgG antibody
类别
资金
- National Natural Science Foundation of China [21904117]
- Zhejiang Provincial Natural Science Foundation of China [LY20B050007, LY21B050003]
- Key Research and Development Program of Guangxi [AB18126033]
In this study, a novel solid-state electrochemiluminescence (ECL) platform was developed for rapid and ultra-sensitive detection of SARS-CoV-2 antibody. The platform utilizes bipolar silica nanochannel array to stably confine the ECL probe and achieve reliable detection of SARS-CoV-2 IgG.
Ultrasensitive, specific, and early identification of Coronavirus Disease (2019) (COVID-19) infection is critical to control virus spread and remains a global public health problem. Herein, we present a novel solid-state electrochemiluminescence (ECL) platform targeting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibody with rapidity and ultrahigh sensitivity, in which a bipolar silica nanochannel array (bp-SNA) is fabricated on indium tin oxide (ITO) electrode for the first time to stably confine the ECL probe of tris(2,2 '-bipyridyl) ruthenium (Ru(bpy)(3)(2+)) under dual electrostatic force. The bp-SNA consists of tightly packed bilayer silica nanochannel array (SNA) with asymmetric surface charges, namely an inner negatively charged SNA (n-SNA) and an outer positively charged SNA (p-SNA), serving as an electrostatic lock to enrich and stabilize the cationic Ru(bpy)(3)(2+) probe without leakage from the electrode surface. The detection of SARS-CoV-2 IgG antibody could be realized via immobilization of SARS-CoV-2 spike protein on the utmost of Ru(bpy)(3)(2+)-confined solid-state ECL platform (Ru@bp-SNA). Upon the capture of target SARS-CoV-2 IgG by immune recognition, the formed immunocomplex will block the nanochannel, leading to the hindered diffusion of the co-reactant (tri-n-propylamine, TPrA) and further producing a decreased ECL signal. The developed solid-stated ECL immunosensor is able to determine SARS-CoV-2 IgG with a wide linear range (5 pg mL(-1) to 1 mu g mL(-1)), a low limit-ofdetection (2.9 pg mL(-1)), and a short incubation time (30 min). Furthermore, accurate analysis of SARS-CoV-2 IgG in real serum samples is also obtained by the sensor.
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