期刊
BIOSENSORS & BIOELECTRONICS
卷 213, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2022.114454
关键词
COVID-19; SARS-CoV-2; Influenza; Adenovirus; Respiratory syncytial virus; Microfluidics; Immunoassay
类别
资金
- MCIN/AEI [PID2019-110713RB-I00]
- ERDF A way of making Europe
- grant DOGV [8815]
- Generalitat Valenciana [PROMETEO/2020/094, 88/2021]
- [PAID-19-01-06]
- [FPI-UPV 2017]
The COVID-19 pandemic has increased the demand for rapid and reliable point-of-care testing devices. This study presents an all-in-one POCT device that can perform parallel viral antigen and serological analysis. The biosensor demonstrates high diagnostic sensitivity and specificity for various respiratory viruses.
The impact of the COVID-19 pandemic has reinforced the need for rapid, cost-effective, and reliable point-of-care testing (POCT) devices for massive population screening. The co-circulation of SARS-CoV-2 with several seasonal respiratory viruses highlights the need for multiplexed biosensing approaches. Herein, we present a fast and robust all-in-one POCT device for parallel viral antigen and serological analysis. The biosensing approach consists of a functionalized polycarbonate disc-shaped surface with microfluidic structures, where specific bioreagents are immobilized in microarray format, and a portable optoelectronic analyzer. The biosensor quantifies the concentration of viral antigens and specific immunoglobulins G and M for SARS-CoV-2, influenza A/B, adenovirus, and respiratory syncytial virus, using 30 mu L. of a sample. The semi-automated analysis of 6 samples is performed in 30 min. Validation studies performed with 135 serum samples and 147 nasopharyngeal specimens reveal high diagnostic sensitivity (98-100%) and specificity (84-98%), achieving an excellent agreement (kappa = 0.937) with commercial immunoassays, which complies with the World Health Organization criteria for POC COVID-19 diagnostic tests. The versatility of the POCT device paves the way for the detection of other pathogens and analytes in the incoming post-pandemic world, integrating specific bioreagents against different variants of concerns and interests.
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