4.8 Article

Rapid screening of antimicrobial probiotics using CRISPR cascade

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Biophysics

Exploiting the orthogonal CRISPR-Cas12a/Cas13a trans-cleavage for dual-gene virus detection using a handheld device

Tian Tian et al.

Summary: This study introduces a highly efficient dual-gene detection technique based on the orthogonal DNA/RNA collateral cleavage mechanism of the Cas12a/Cas13a system. The technique allows simultaneous detection of multiple amplified products and can be implemented on a portable device for accurate infectious disease screening in resource-limited settings.

BIOSENSORS & BIOELECTRONICS (2022)

Article Chemistry, Multidisciplinary

PAM-less conditional DNA substrates leverage trans-cleavage of CRISPR-Cas12a for versatile live-cell biosensing

Siyu Chen et al.

Summary: In this study, the CRISPR-Cas system was engineered as a versatile live-cell biosensing system for monitoring diverse intracellular biomolecules. By leveraging the trans-cleavage activity of CRISPR-Cas12a, this system enabled sensitive sensing of various biomolecules such as telomerase, ATP, and microRNA-21.

CHEMICAL SCIENCE (2022)

Article Biophysics

Paper-supported near-infrared-light-triggered photoelectrochemical platform for monitoring Escherichia coli O157:H7 based on silver nanoparticles-sensitized-upconversion nanophosphors

Mingming Yin et al.

Summary: In this study, a near-infrared-responsive photoelectrochemical sensing platform was developed for ultrasensitive detection of E. coli O157:H7. By assembling different nanomaterials, the sensor achieved specific capture and determination of E. coli with the recognition element of an antimicrobial peptide.

BIOSENSORS & BIOELECTRONICS (2022)

Article Chemistry, Analytical

CRISPR/Cas12a-based biosensor for colorimetric detection of serum prostate-specific antigen by taking nonenzymatic and isothermal amplification

Wanhe Wang et al.

Summary: CRISPR/Cas12a-based biosensors have been widely studied for nucleic acid detection, but this study demonstrates their use in colorimetric detection of serum PSA using an enzyme-free HCR-powered system. The assay shows sensitive and selective detection capabilities in both visual and quantitative modes, highlighting its potential for protein cancer biomarker detection.

SENSORS AND ACTUATORS B-CHEMICAL (2022)

Article Biophysics

A switchable Cas12a enabling CRISPR-based direct histone deacetylase activity detection

Wenyuan Kang et al.

Summary: The study demonstrates the use of a switchable Cas12a for direct analysis of histone deacetylase (HDAC) activity. The inactivation of Cas12a by acetylation can be reversed by HDAC-mediated deacetylation. By leveraging this switchable Cas12a, a sensitive one-pot assay for detecting HDAC activity was developed, with sub-nanomolar sensitivity. This assay can be applied in biochemical studies and clinical diagnostics.

BIOSENSORS & BIOELECTRONICS (2022)

Article Biochemistry & Molecular Biology

Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy

Parinaz Fozouni et al.

Summary: This study developed an amplification-free CRISPR-Cas13a assay for direct detection of SARS-CoV-2, showing rapid, highly sensitive, and mobile phone-based readout capabilities.
Article Biophysics

Light-up RNA aptamer signaling-CRISPR-Cas13a-based mix-and-read assays for profiling viable pathogenic bacteria

Ting Zhang et al.

Summary: This study developed a new detection system that can accurately distinguish viable pathogenic bacteria without the need for reverse transcription, nucleic acid amplification, or labeled substrates. By utilizing CRISPR-Cas13a and a light-up RNA aptamer, it enables rapid detection and precise quantification of viable pathogens.

BIOSENSORS & BIOELECTRONICS (2021)

Review Microbiology

Adaptations of Vibrio parahaemolyticus to Stress During Environmental Survival, Host Colonization, and Infection

Gururaja Perumal Pazhani et al.

Summary: Vibrio parahaemolyticus is a Gram-negative aquatic bacterium that can infect humans, causing gastroenteritis and wound infections. It has evolved several genetic mechanisms to adapt to survive in the environment and human host, exhibiting strong virulence. Vp possesses multiple secretion systems that induce cytotoxicity and enterotoxicity in the human body.

FRONTIERS IN MICROBIOLOGY (2021)

Article Chemistry, Analytical

Duplex-Specific Nuclease-Assisted CRISPR-Cas12a Strategy for MicroRNA Detection Using a Personal Glucose Meter

Shaohua Gong et al.

Summary: This study presents a novel CRISPR-Cas12a strategy using duplex-specific nuclease and a personal glucose meter for miRNA detection, avoiding expensive equipment and demonstrating high sensitivity and good selectivity.

ANALYTICAL CHEMISTRY (2021)

Article Chemistry, Multidisciplinary

Controlling Gene Expression in Mammalian Cells Using Multiplexed Conditional Guide RNAs for Cas12a**

Lukas Oesinghaus et al.

Summary: The study demonstrates that conditional guide RNAs (gRNAs) for Cas12a can be transcribed in mammalian cells by RNA polymerase II and activated via input-dependent processing of the 3' tail of the gRNA transcript, providing a new route towards Cas12a-based gene regulation constructs. The principle has been shown to work in HEK and mouse fibroblast cells using luminescence, fluorescence, and for the conditional upregulation of an endogenous gene.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2021)

Review Chemistry, Multidisciplinary

CRISPR technology incorporating amplification strategies: molecular assays for nucleic acids, proteins, and small molecules

Wei Feng et al.

Summary: The advancement of CRISPR-Cas technology has revolutionized genome editing and transcriptional modulation, as well as molecular detection of diverse targets. Integrating CRISPR-Cas systems with nucleic acid amplification strategies enables amplified detection signals, enrichment of low-abundance targets, improved specificity and sensitivity, and development of point-of-care diagnostic techniques.

CHEMICAL SCIENCE (2021)

Article Chemistry, Physical

Biosurfactants, natural alternatives to synthetic surfactants: Physicochemical properties and applications

Ruksana Jahan et al.

ADVANCES IN COLLOID AND INTERFACE SCIENCE (2020)

Review Food Science & Technology

Interactions between probiotics and pathogenic microorganisms in hosts and foods: A review

Amin Mousavi Khaneghah et al.

TRENDS IN FOOD SCIENCE & TECHNOLOGY (2020)

Article Chemistry, Multidisciplinary

Single-Step, Salt-Aging-Free, and Thiol-Free Freezing Construction of AuNP-Based Bioprobes for Advancing CRISPR-Based Diagnostics

Menglu Hu et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2020)

Article Multidisciplinary Sciences

Ultrasensitive CRISPR-based diagnostic for field-applicable detection of Plasmodium species in symptomatic and asymptomatic malaria

Rose A. Lee et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2020)

Review Food Science & Technology

Crop biotechnology and the future of food

Michael A. Steinwand et al.

NATURE FOOD (2020)

Article Infectious Diseases

Impact of antimicrobial therapy on the gut microbiome

Amira A. Bhalodi et al.

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY (2019)

Review Biotechnology & Applied Microbiology

Quorum sensing inhibitors as antipathogens: biotechnological applications

Vipin Chandra Kalia et al.

BIOTECHNOLOGY ADVANCES (2019)

Review Infectious Diseases

Antibiotic discovery: history, methods and perspectives

Guillaume Andre Durand et al.

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS (2019)

Review Microbiology

Biofilm-associated infection by enterococci

Jun-Hong Ch'ng et al.

NATURE REVIEWS MICROBIOLOGY (2019)

Article Multidisciplinary Sciences

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

Janice S. Chen et al.

SCIENCE (2018)

Article Multidisciplinary Sciences

Pathogen elimination by probiotic Bacillus via signalling interference

Pipat Piewngam et al.

NATURE (2018)

Article Chemistry, Multidisciplinary

Temperature-Robust DNAzyme Biosensors Confirming Ultralow Background Detection

Ruijie Deng et al.

ACS SENSORS (2018)

Review Food Science & Technology

Short chain and polyunsaturated fatty acids in host gut health and foodborne bacterial pathogen inhibition

Mengfei Peng et al.

CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION (2017)

Article Multidisciplinary Sciences

Nucleic acid detection with CRISPR-Cas13a/C2c2

Jonathan S. Gootenberg et al.

SCIENCE (2017)

Review Biotechnology & Applied Microbiology

Antibiotic resistance of Vibrio parahaemolyticus and Vibrio vulnificus in various countries: A review

Sara Elmahdi et al.

FOOD MICROBIOLOGY (2016)

Article Agriculture, Dairy & Animal Science

Production of bacteriocin-like inhibitory substance by Bifidobacterium lactis in skim milk supplemented with additives

Fabio Andres Castillo Martinez et al.

JOURNAL OF DAIRY RESEARCH (2015)