Cellular and structural insight into dynamin function during endocytic vesicle formation: a tale of 50 years of investigation

Dynamin is one of the major proteins involved in endocytosis. First identified 50 years ago in a genetic screen in Drosophila melanogaster, it has become a central player in many forms of endocytosis, such as clathrin-mediated endocytosis or synaptic vesicle endocytosis, as well as other important cellular processes such as actin remodelling. Decades of work using biochemical and structural studies, cell-free assays, live cell imaging, acute inhibition and genetic studies have led to important insights on its mode of action. Dynamin is a remarkable mechano-GTPase, which can do a lot to membranes on its own but which is, in cells, at the centre of a vast protein and lipid network and cannot work in isolation. This review summarizes the main features of dynamin structure and function and its central role in membrane remodelling events, and give an update on the latest results.

Automated summary

Dynamin is a major protein involved in endocytosis and plays a central role in cellular processes such as actin remodeling. It has been extensively studied through various methods and has been shown to be a remarkable mechano-GTPase. It is part of a complex protein and lipid network in cells and cannot function in isolation.