Metagenomics reveals the metabolism of polyphosphate-accumulating organisms in biofilm sequencing batch reactor: A new model

This study assessed the impact of the operating conditions of the biofilm sequencing batch reactor (BSBR) on the community structure and the growth/metabolic pathways of its polyphosphate-accumulating organisms (PAOs). There are significant difference with reference to the enhanced biological phosphorus removal (EBPR) process. The leading PAOs in BSBR generally are capable of high affinity acetate metabolism, gluconeogenesis, and low affinity phosphate transport, and have various carbon source supplementation pathways to ensure the efficient circulation of energy and reducing power. A new model of the metabolic mechanism of PAOs in the BSBR was formulated, which features low glycogen metabolism with simultaneous gluconeogenesis and glycogenolysis and differs significantly from the classic mechanism based on Candidatus_Accumulibacter and Tetrasphaera. The findings will assist the efficient recovery of low concentration phosphate in municipal wastewater.

Automated summary

This study assessed the impact of operating conditions of a biofilm sequencing batch reactor (BSBR) on the community structure and metabolic pathways of polyphosphate-accumulating organisms (PAOs). The findings suggest significant differences compared to the enhanced biological phosphorus removal (EBPR) process. The PAOs in BSBR have various metabolic pathways to ensure efficient energy circulation and show distinct differences from the classic mechanism based on Candidatus_Accumulibacter and Tetrasphaera.