Enhancement of separation selectivity of hemicellulose from bamboo using freeze-thaw-assisted p-toluenesulfonic acid treatment at low acid concentration and high temperature

The cellulose-rich residual solids are obtained with p-toluenesulfonic acid (p-TsOH) treatment. However, better fractionation of hemicellulose and separation is difficult to obtain during treatment. This study aims at inves-tigating the separation selectivity of bamboo hemicellulose using freeze-thaw-assisted p-TsOH (F/p-TsOH) treatment. The desired separation effect was achieved at freezing temperature -40 ?C, freezing time 20 h, p-TsOH concentration 3.0 %, treatment temperature 130 ?C and time 80 min. 93.26 % hemicellulose separation was found, which was 32.88 % higher than that of conventional p-TsOH treatment. Furthermore, the separation yield of lignin decreased significantly from 69.29 % to 13.98 %. The distinct lignin characteristic absorption peaks were found, while that of hemicellulose was difficult to observe. The fiber crystallinity index increased from 50.42 to 56.55 %. Furthermore, greater selectivity for hemicellulose separation was achieved. The results provide a new research thinking for efficient fractionation of lignocellulosic biomass by organic acid treatment.

Automated summary

This study achieved efficient separation of bamboo hemicellulose through freeze-thaw-assisted p-TsOH treatment. Compared to conventional methods, better separation effect was achieved with significantly reduced yield of lignin. In addition, an increase in fiber crystallinity index and higher separation selectivity were observed.