Deciphering the spatial distribution along the upflow anammox reactor: Sludge characteristics and interspecies interactions

Here, nitrogen conversion, granular characteristics and microbial dynamics were combined to reveal the longitudinal heterogeneity along anammox-UASB with nitrogen removal efficiency of 92.6%. The reactor was divided into Bottom-zone, Middle-zone, Upper-zone, and Top-zone with height increasing. Results indicated that particle size decreased from Bottom-zone to Upper-zone, while granular floatation caused an increase in Top-zone. Protein secondary structure in EPS was loose and hzsA transcription ratio was only 4.45% due to the limited mass-transfer and serious mineralization of ultra-large granules in Bottom-zone. Smaller granules in Middle-zone were more robust and active, with compact tryptophan- and aromatic-like protein in EPS and 23.71% hzsA transcription. Intriguingly, coexisting denitrification survived on EPS and/or microbial metabolites was observed. Transcription of narG was stimulated with height increasing, resulted in performance improvement through combining partial denitrification and anammox in Upper-zone. The findings deciphered stratification characteristics along the height-partitioned anammox-UASB, and reveal cross-feedings between denitrification and anammox bacteria.

Automated summary

This study combined nitrogen conversion, granular characteristics, and microbial dynamics to reveal the longitudinal heterogeneity of anammox-UASB. It was found that the bottom zone had larger and more mineralized granules, while the middle zone had smaller and more active granules. By combining partial denitrification and anammox, the performance in the top zone was improved.