期刊
BIORESOURCE TECHNOLOGY
卷 361, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2022.127690
关键词
3-Hydroxypropionate; Metabolic transforming; Malonyl-CoA reductase; Plasmid loss; Chassis cell
资金
- National Key Research and Development Program of China [2018YFA0900300]
- National Natural Science Foundation of China [21877111]
- Innovation grant of Dalian Institute of Chemical Physics [DICP I201920]
In this study, the production efficiency of 3-HP was improved by optimizing gene expression and providing sufficient precursor and cofactor supply. The highest 3-HP production from glucose was achieved.
3-Hydroxypropionate (3-HP) is a platform chemical for production of acrylic acid, acrylamide and biodegradable polymers. Several microbial cell factories have been constructed for production of 3-HP from malonyl-CoA by using a malonyl-CoA reductase, which however suffer from inadequate supply of precursor and cofactor. Here 3 -HP biosynthesis was optimized in a super yeast chassis with sufficient supply of precursor malonyl-CoA and cofactor NADPH, which had a 3-fold higher 3-HP (1.4 g/L) than that of wild-type background. The instability of the engineered strain was observed in fed-batch fermentation due to the plasmid loss, which may be caused by the toxic intermediate malonate semialdehyde. Genome integration of MCR-C encoding C-terminal of MCR enabled stable gene expression and much higher 3-HP production of 4.4 g/L under batch fermentation and 56.5 g/L under fed-batch fermentation with a yield of 0.31 g/g glucose. This was the highest 3-HP production re-ported from glucose in engineered microbes.
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