1H, 13C, 15N resonance assignment of the enzyme KdgF from Bacteroides eggerthii

To fully utilize carbohydrates from seaweed biomass, the degradation of the family of polysaccharides known as alginates must be understood. A step in the degradation of alginate is the conversion of 4,5-unsaturated monouronates to 4-deoxy-L-erythro-5-hexoseulose catalysed by the enzyme KdgF. In this study BeKdgF from Bacteroides eggerthii from the human gut microbiota has been produced isotopically labelled in Escherichia coli. Here the H-1, C-13, and N-15 NMR chemical shift assignment for BeKdgF is reported.

Automated summary

This study reports the isotopically labeled BeKdgF from Bacteroides eggerthii from the human gut microbiota and the assignment of its H-1, C-13, and N-15 NMR chemical shifts.