期刊
BIOMOLECULAR NMR ASSIGNMENTS
卷 16, 期 2, 页码 343-347出版社
SPRINGER
DOI: 10.1007/s12104-022-10102-6
关键词
KdgF; Alginate degradation; 4-deoxy-L-erythro-5-hexoseulose (DEH); Human gut microbiota
资金
- NTNU Norwegian University of Science and Technology (St. Olavs Hospital -Trondheim University Hospital)
- Norwegian Research Council (NFR) [315385, 226244]
- Innovationsfonden [1308-00011B]
This study reports the isotopically labeled BeKdgF from Bacteroides eggerthii from the human gut microbiota and the assignment of its H-1, C-13, and N-15 NMR chemical shifts.
To fully utilize carbohydrates from seaweed biomass, the degradation of the family of polysaccharides known as alginates must be understood. A step in the degradation of alginate is the conversion of 4,5-unsaturated monouronates to 4-deoxy-L-erythro-5-hexoseulose catalysed by the enzyme KdgF. In this study BeKdgF from Bacteroides eggerthii from the human gut microbiota has been produced isotopically labelled in Escherichia coli. Here the H-1, C-13, and N-15 NMR chemical shift assignment for BeKdgF is reported.
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