4.7 Article

Guided interactive image segmentation using machine learning and color-based image set clustering

期刊

BIOINFORMATICS
卷 38, 期 19, 页码 4622-4628

出版社

OXFORD UNIV PRESS
DOI: 10.1093/bioinformatics/btac547

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资金

  1. German Federal Ministry of Education and Research (BMBF) [031L0257J, 031L0256C, 031L0035]
  2. German Research Foundation (DFG) [HO 4772/1-1]

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In this paper, a novel approach combining machine learning and clustering methods is presented for interactive segmentation and quantification of large image sets. The approach addresses the issue of decreased accuracy when reusing classifiers in biological and medical images due to color variability and improves the applicability and efficiency of interactive segmentation.
Motivation: Over the last decades, image processing and analysis have become one of the key technologies in systems biology and medicine. The quantification of anatomical structures and dynamic processes in living systems is essential for understanding the complex underlying mechanisms and allows, i.e. the construction of spatio-temporal models that illuminate the interplay between architecture and function. Recently, deep learning significantly improved the performance of traditional image analysis in cases where imaging techniques provide large amounts of data. However, if only a few images are available or qualified annotations are expensive to produce, the applicability of deep learning is still limited. Results: We present a novel approach that combines machine learning-based interactive image segmentation using supervoxels with a clustering method for the automated identification of similarly colored images in large image sets which enables a guided reuse of interactively trained classifiers. Our approach solves the problem of deteriorated segmentation and quantification accuracy when reusing trained classifiers which is due to significant color variability prevalent and often unavoidable in biological and medical images. This increase in efficiency improves the suitability of interactive segmentation for larger image sets, enabling efficient quantification or the rapid generation of training data for deep learning with minimal effort. The presented methods are applicable for almost any image type and represent a useful tool for image analysis tasks in general.

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